Jashari Besart, Capitaine Karine, Bisha Bledar, Stessl Beatrix, Blagoevska Katerina, Cana Armend, Jankuloski Dean, Félix Benjamin
Food Microbiology, Food and Veterinary Laboratory, Food and Veterinary Agency of Kosovo, Lidhja e Pejës 241, 10000 Pristina, Kosovo.
Laboratory for Food Safety, Salmonella and Listeria Unit, ANSES, European Union Reference Laboratory for L. monocytogenes, University of Paris-Est, 94700 Maisons-Alfort, France.
Foods. 2024 Sep 12;13(18):2883. doi: 10.3390/foods13182883.
The present study describes the genetic characterization of strains found in the Republic of Kosovo's food chain. From 2016 to 2022, 995 samples were collected. Overall, 648 samples were from ready-to-eat (RTE) food products, 281 from food products consumed cooked (FPCC), 60 from raw materials, and 6 from environmental samples. Overall, 11.76% (117 out of 995) of the samples were contaminated by , comprising 6.33% (41 out of 648) from RTE products, 14.95% (42 out of 281) from FPCC, 55.00% (33 out of 60) from raw materials, and 16.66% (1 out of 6) from environmental samples. All isolates were subjected to molecular serotyping and clonal complex (CC) identification by using real-time PCR, as well as multilocus sequence typing. All isolates were grouped into four molecular serotypes, IIa (34.19%), IIb (3.48%), IIc (32.48%), and IVb (29.91%), as well as Lineage I (33.33%) and Lineage II (66.66%). In total, 14 CCs were identified from 41 RTE isolates; however, CC29 (7), CC2 (6), and CC6 (6) were the most dominant. By contrast, CC9 was by far the most represented CC in both FPCC (21) and RM (14). Moreover, 30 isolates expressed CC1, CC2, CC4, or CC6, which are particularly associated with severe human infections.
本研究描述了在科索沃共和国食物链中发现的菌株的基因特征。2016年至2022年期间，共采集了995份样本。总体而言，648份样本来自即食（RTE）食品，281份来自熟食消费食品（FPCC），60份来自原材料，6份来自环境样本。总体而言，11.76%（995份样本中的117份）的样本被污染，其中RTE产品占6.33%（648份中的41份），FPCC占14.95%（281份中的42份），原材料占55.00%（60份中的33份），环境样本占16.66%（6份中的1份）。所有分离株均通过实时PCR以及多位点序列分型进行分子血清分型和克隆复合体（CC）鉴定。所有分离株被分为四种分子血清型，IIa（34.19%）、IIb（3.48%）、IIc（32.48%）和IVb（29.91%），以及谱系I（33.33%）和谱系II（66.66%）。从41份RTE分离株中总共鉴定出14个CC；然而，CC29（7个）、CC2（6个）和CC6（6个）最为常见。相比之下，CC9是FPCC（21个）和RM（14个）中迄今为止最具代表性的CC。此外，30个分离株表达CC1、CC2、CC4或CC6，这些与严重的人类感染特别相关。
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