Zhang Hongzhi, Chen Weijie, Wang Jing, Xu Biyao, Liu Hong, Dong Qingli, Zhang Xi
Shanghai Municipal Center for Disease Control and Prevention, Shanghai, China.
Minhang District Center for Disease Control and Prevention, Shanghai, China.
Front Microbiol. 2020 Dec 15;11:551020. doi: 10.3389/fmicb.2020.551020. eCollection 2020.
is an etiologic agent of listeriosis, and has emerged as an important foodborne pathogen worldwide. In this study, the molecular characteristics of 155 isolates from seven food groups in Shanghai, the biggest city in China, were identified using whole-genome sequencing (WGS). Most isolates (79.3%) were obtained between May and October from 2009 to 2019. The serogroups and clonal complexes (CCs) of were found useful for identifying potential health risks linked to foods. Differences in distributions of serogroups and CCs among different food groups were analyzed using -test. The results showed that the IIa and IVb serogroups were identified among most of food groups. However, the prevalence of serogroup IIb was significantly higher in ready-to-eat (RTE) food and raw seafood than in other food groups, similar to group IIc in raw meat and raw poultry than others. Meanwhile, the prevalence of CC9 in raw meat and raw poultry, CC8 in raw poultry, and CC87 in raw seafood significantly exceeded that of in other food groups. Specially, CC87 was the predominant CC in foodborne and clinical isolates in China, indicating that raw seafood may induce a high-risk to food safety. Also, hypervirulence pathogenicity islands LIPI-3 and LIPI-4 were found in CC3, CC1, and CC87, respectively. The clonal group CC619 carried LIPI-3 and LIPI-4, as previously reported in China. Core genome multilocus sequence typing (cgMLST) analysis suggested that CC87 isolates from the same food groups in different years had no allelic differences, indicating that could persist over years. These 10-year results in Shanghai underscore the significance of molecular epidemiological surveillance of in foodborne products in assessing the potential risk of this pathogen, and further address food safety issues in China.
是李斯特菌病的病原体，已成为全球重要的食源性病原体。在本研究中，使用全基因组测序（WGS）鉴定了来自中国最大城市上海七个食品组的155株分离株的分子特征。大多数分离株（79.3%）于2009年至2019年的5月至10月间获得。发现其血清群和克隆复合体（CCs）有助于识别与食品相关的潜在健康风险。使用卡方检验分析不同食品组之间血清群和CCs分布的差异。结果表明，大多数食品组中都鉴定出了IIa和IVb血清群。然而，即食（RTE）食品和生海鲜中血清群IIb的流行率显著高于其他食品组，生肉和生禽中血清群IIc的流行率高于其他食品组。同时，生肉和生禽中CC9、生禽中CC8和生海鲜中CC87的流行率显著高于其他食品组。特别地，CC87是中国食源和临床分离株中的主要CC，表明生海鲜可能对食品安全构成高风险。此外，在CC3、CC1和CC87中分别发现了高毒力致病岛LIPI-3和LIPI-4。如先前在中国报道的那样，克隆组CC619携带LIPI-3和LIPI-4。核心基因组多位点序列分型（cgMLST）分析表明，不同年份来自同一食品组的CC87分离株没有等位基因差异，表明可在多年间持续存在。上海这10年的结果强调了对食源产品中进行分子流行病学监测在评估该病原体潜在风险方面的重要性，并进一步解决了中国的食品安全问题。
