School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China.
J Proteomics. 2012 Oct 22;75(18):5604-27. doi: 10.1016/j.jprot.2012.07.041. Epub 2012 Aug 3.
Nostoc flagelliforme must undergo a dehydration/rehydration cycle during its growth stages; the mechanisms underlying this constraint are examined. The novel insights into N. flagelliforme's response to desiccation and rehydration at ultrastructural, physiological and proteomic levels were offered. The structure of colonies and cells remained unchanged in response to dehydration and rehydration treatments except that the sheath appeared shrunken, and both the quantity and volume of vacuoles were decreased when dehydrated compared with rehydration. A significant increase in photosynthesis, respiration, total Rubisco activity, superoxide anion level, SOD, CAT, POD, nitrogenase and glutamine synthetase (GS) activities in response to rehydration was noted, whereas H(2)O(2), ammonium, proline and glutamate contents all registered a decrease. 32 differentially expressed proteins between dehydrated and rehydrated colonies were categorized according to their predicted functions into secretion, signaling, transcription and translation, antioxidative processes, nitrogen metabolism, energy metabolism, lipid metabolism and chaperonin. The dehydration is a quiescent state in which metabolism is down-regulated, upon rehydration, a metabolic shift occurs from quiescent to active. The specific metabolic and regulated mechanisms to accommodate the dehydration/rehydration cycle in N. flagelliforme is reported here.
发菜在生长阶段必须经历脱水/复水循环；研究了这种限制的机制。提供了关于发菜在超微结构、生理和蛋白质组水平上对干燥和复水响应的新见解。除了鞘看起来收缩之外，菌落和细胞的结构在脱水和复水处理下保持不变，并且与复水相比，当脱水时，液泡的数量和体积减少。在复水时，光合作用、呼吸作用、总 Rubisco 活性、超氧阴离子水平、SOD、CAT、POD、固氮酶和谷氨酰胺合成酶 (GS) 活性显著增加，而 H(2)O(2)、铵、脯氨酸和谷氨酸含量均下降。根据预测功能，将脱水和复水菌落之间的 32 种差异表达蛋白分为分泌、信号、转录和翻译、抗氧化过程、氮代谢、能量代谢、脂质代谢和伴侣蛋白。脱水是一种休眠状态，代谢受到抑制，复水后，代谢从休眠到活跃发生转变。本文报道了发菜适应脱水/复水循环的特定代谢和调节机制。
