Patamia Vincenzo, Zagni Chiara, Fiorenza Roberto, Fuochi Virginia, Dattilo Sandro, Riccobene Paolo Maria, Furneri Pio Maria, Floresta Giuseppe, Rescifina Antonio
Dipartimento di Scienze del Farmaco e della Salute, Università di Catania, Viale A. Doria 6, 95125 Catania, Italy.
Dipartimento di Scienze Chimiche, Università di Catania, Viale A. Doria 6, 95125 Catania, Italy.
Nanomaterials (Basel). 2023 Jul 10;13(14):2036. doi: 10.3390/nano13142036.
Bacterial involvement in cancer's development, along with their impact on therapeutic interventions, has been increasingly recognized. This has prompted the development of novel strategies to disrupt essential biological processes in microbial cells. Among these approaches, metal-chelating agents have gained attention for their ability to hinder microbial metal metabolism and impede critical reactions. Nanotechnology has also contributed to the antibacterial field by offering various nanomaterials, including antimicrobial nanoparticles with potential therapeutic and drug-delivery applications. Halloysite nanotubes (HNTs) are naturally occurring tubular clay nanomaterials composed of aluminosilicate kaolin sheets rolled multiple times. The aluminum and siloxane groups on the surface of HNTs enable hydrogen bonding with biomaterials, making them versatile in various domains, such as environmental sciences, wastewater treatment, nanoelectronics, catalytic studies, and cosmetics. This study aimed to create an antibacterial material by combining the unique properties of halloysite nanotubes with the iron-chelating capability of kojic acid. A nucleophilic substitution reaction involving the hydroxyl groups on the nanotubes' surface was employed to functionalize the material using kojic acid. The resulting material was characterized using infrared spectroscopy (IR), thermogravimetric analysis (TGA), energy-dispersive X-ray spectroscopy (EDX), and scanning electron microscopy (SEM), and its iron-chelating ability was assessed. Furthermore, the potential for drug loading-specifically, with resveratrol and curcumin-was evaluated through ultraviolet (UV) analysis. The antibacterial assay was evaluated following CLSI guidelines. The results suggested that the HNTs-kojic acid formulation had great antibacterial activity against all tested pathogens. The outcome of this work yielded a novel bio-based material with dual functionality as a drug carrier and an antimicrobial agent. This innovative approach holds promise for addressing challenges related to bacterial infections, antibiotic resistance, and the development of advanced therapeutic interventions.
细菌在癌症发展中的作用及其对治疗干预的影响已得到越来越多的认识。这促使人们开发新的策略来破坏微生物细胞中的基本生物过程。在这些方法中，金属螯合剂因其能够阻碍微生物金属代谢并阻止关键反应而受到关注。纳米技术也通过提供各种纳米材料为抗菌领域做出了贡献，包括具有潜在治疗和药物递送应用的抗菌纳米颗粒。埃洛石纳米管（HNTs）是由铝硅酸盐高岭土片多次卷曲而成的天然管状粘土纳米材料。HNTs表面的铝和硅氧烷基团能够与生物材料形成氢键，使其在环境科学、废水处理、纳米电子学、催化研究和化妆品等各个领域都具有通用性。本研究旨在通过将埃洛石纳米管的独特性能与曲酸的铁螯合能力相结合，制备一种抗菌材料。利用涉及纳米管表面羟基的亲核取代反应，用曲酸对材料进行功能化。使用红外光谱（IR）、热重分析（TGA）、能量色散X射线光谱（EDX）和扫描电子显微镜（SEM）对所得材料进行表征，并评估其铁螯合能力。此外，通过紫外（UV）分析评估了药物负载的潜力，特别是白藜芦醇和姜黄素的负载潜力。按照CLSI指南进行抗菌试验评估。结果表明，HNTs-曲酸制剂对所有测试病原体都具有很强的抗菌活性。这项工作的成果产生了一种具有双重功能的新型生物基材料，可作为药物载体和抗菌剂。这种创新方法有望应对与细菌感染、抗生素耐药性以及先进治疗干预措施开发相关的挑战。
