Yu Zijie, He Xinhua, Li Zhitong, Zhou Shuang, Guo Dalu, Pu Hao, Luo Hongyan
College of Resources and Environment, Southwest University, Chongqing, China.
Front Microbiol. 2023 Jan 23;14:1098681. doi: 10.3389/fmicb.2023.1098681. eCollection 2023.
Anaerobic ammonium oxidation (anammox) plays a vital role in the global nitrogen cycle by oxidizing ammonium to nitrogen under anaerobic environments. However, the existence, abundance, and diversity of anammox bacteria between different temperatures are less studied, particularly in purple paddy soils.
C-DNA stable-isotope probe combined with Illumina MiSeq high-throughput sequencing was employed to explore soil abundance and diversity of anammox bacteria. In doing so, 40-60 cm depth soils from typical purple paddy soils in Chongqing, southwest China, were cultured under CO-labeled and CO-labeled at 35°C, 25°C, 15°C, and 5°C for 56 days.
Anammox bacteria were not labeled at all by CO at 5°C. The highest abundance of anammox bacteria was found at 25°C (3.52 × 103.66 × 10 copies·g dry soil), followed by 35°C and 15°C (2.01 × 102.37 × 10 copies·g dry soil) and almost no increase at 5°C. The relative abundance of sp. was higher at 25°C and 15°C, while sp. was higher at 35°C and 5°C. Our results revealed differences in anammox bacteria at different temperatures in purple paddy soils, which could provide a better understanding of soil N cycling regulated by anammox bacteria.
厌氧氨氧化在厌氧环境中将铵氧化为氮气，在全球氮循环中发挥着至关重要的作用。然而，不同温度下厌氧氨氧化细菌的存在、丰度和多样性研究较少，尤其是在紫色水稻土中。
采用C-DNA稳定同位素探针结合Illumina MiSeq高通量测序技术，探究厌氧氨氧化细菌的土壤丰度和多样性。为此，取自中国西南部重庆典型紫色水稻土40 - 60厘米深度的土壤，在35°C、25°C、15°C和5°C下分别用¹³CO₂标记和¹²CO₂标记培养56天。
在5°C时，¹³CO₂完全没有标记到厌氧氨氧化细菌。厌氧氨氧化细菌丰度最高出现在25°C（3.52×10⁶3.66×10⁶拷贝·克干土），其次是35°C和15°C（2.01×10⁶2.37×10⁶拷贝·克干土），在5°C时几乎没有增加。某菌属的相对丰度在25°C和15°C时较高，而另一菌属在35°C和5°C时较高。我们的结果揭示了紫色水稻土中不同温度下厌氧氨氧化细菌的差异，这有助于更好地理解厌氧氨氧化细菌对土壤氮循环的调控。
