Northwestern University, Evanston, IL 60208, USA.
Nanomedicine (Lond). 2013 Jan;8(1):17-28. doi: 10.2217/nnm.12.82. Epub 2012 Aug 14.
A novel biomimetic strategy was employed for presenting antibodies on gold nanorods (NRs) to target growth factor receptors on cancer cells for use in photothermal therapy.
MATERIALS & METHODS: Polydopamine (PD) was polymerized onto gold NRs, and EGF receptor antibodies (anti-EGFR) were immobilized onto the layer. Cell-binding affinity and light-activated cell death of cancer cells incubated with anti-EGFR-PD-NRs were quantified by optical imaging.
PD was deposited onto gold NRs, and antibodies were bound to PD-coated NRs. Anti-EGFR-PD-NRs were stable in media, and were specifically bound to EGFR-overexpressing cells. Illumination of cells targeted with anti-EGFR-PD-NRs enhanced cell death compared with nonirradiated controls and cells treated with antibody-free NRs.
PD facilitates the surface functionalization of gold NRs with biomolecules, allowing cell targeting and photothermal killing of cancer cells. PD can potentially coat a large variety of nanoparticles with targeting ligands as a strategy for biofunctionalization of diagnostic and therapeutic nanoparticles.
采用一种新的仿生策略，将抗体展示在金纳米棒（NRs）上，以靶向癌细胞上的生长因子受体，用于光热治疗。
聚多巴胺（PD）聚合在金 NRs 上，EGF 受体抗体（抗 EGFR）固定在该层上。通过光学成像定量测定与抗 EGFR-PD-NRs 孵育的癌细胞的细胞结合亲和力和光激活细胞死亡。
PD 沉积在金 NRs 上，抗体结合到 PD 涂层的 NRs 上。抗 EGFR-PD-NRs 在介质中稳定，并且特异性结合 EGFR 过表达的细胞。与未辐照对照和未用抗体处理的 NRs 处理的细胞相比，用抗 EGFR-PD-NRs 靶向的细胞的光照增强了细胞死亡。
PD 促进了金 NRs 表面的生物分子功能化，允许癌细胞的靶向和光热杀伤。PD 可以潜在地将各种靶向配体涂覆在具有靶向配体的大颗粒上，作为诊断和治疗纳米颗粒的生物功能化策略。
