Department of Physiology, School of Veterinary Medicine, Shiraz University , Shiraz ,Islamic Republic of Iran.
Int J Radiat Biol. 2013 Feb;89(2):128-31. doi: 10.3109/09553002.2012.721051. Epub 2012 Sep 7.
This study was conducted to evaluate the effect of radiofrequency wave (RFW)-induced oxidative stress in the eye and the prophylactic effect of vitamin C on this organ by measuring the antioxidant enzymes activity including: glutathione peroxidase (GPx), superoxide dismutase (SOD) and catalase (CAT), and malondialdehyde (MDA).
Thirty-two adult male Sprague-Dawley rats were randomly divided into four experimental groups and treated daily for 45 days as follows: Control, vitamin C (L-ascorbic acid 200 mg/kg of body weight/day by gavage), test (exposed to 900 MHz RFW) and the treated group (received vitamin C in addition to exposure to RFW). At the end of the experiment all animals were sacrificed, their eyes were removed and were used for measurement of antioxidant enzymes and MDA activity.
The results indicate that exposure to RFW in the test group decreased antioxidant enzymes activity and increased MDA compared with the control groups (P < 0.05). In the treated group vitamin C improved antioxidant enzymes activity and reduced MDA compared to the test group (P < 0.05).
It can be concluded that RFW causes oxidative stress in the eyes and vitamin C improves the antioxidant enzymes activity and decreases MDA.
本研究旨在评估射频波（RFW）诱导的眼部氧化应激的影响，以及通过测量抗氧化酶活性，包括谷胱甘肽过氧化物酶（GPx）、超氧化物歧化酶（SOD）和过氧化氢酶（CAT），以及丙二醛（MDA），来评估维生素 C 对该器官的预防作用。
32 只成年雄性 Sprague-Dawley 大鼠被随机分为四组实验，并在 45 天内每天接受以下处理：对照组、维生素 C（L-抗坏血酸 200mg/kg 体重/天灌胃）、测试组（暴露于 900MHz RFW 下）和治疗组（在暴露于 RFW 之外还接受维生素 C 治疗）。实验结束时，所有动物被处死，取出眼睛，用于测量抗氧化酶和 MDA 活性。
结果表明，与对照组相比，测试组暴露于 RFW 后抗氧化酶活性降低，MDA 增加（P<0.05）。在治疗组中，与测试组相比，维生素 C 提高了抗氧化酶活性并降低了 MDA（P<0.05）。
可以得出结论，RFW 会导致眼部氧化应激，而维生素 C 可以提高抗氧化酶活性并降低 MDA。
