D'Silva Mary Hydrina, Swer Rijied Thompson, Anbalagan J, Rajesh Bhargavan
Tutor, Department of Anatomy, Andaman and Nicobar Islands Institute of Medical Sciences, Port Blair, India.
Associate Professor, Department of Anatomy, Andaman and Nicobar Islands Institute of Medical Sciences, Port Blair, India.
J Clin Diagn Res. 2017 Jul;11(7):AC05-AC09. doi: 10.7860/JCDR/2017/26360.10275. Epub 2017 Jul 1.
The increasing scientific evidence of various health hazards on exposure of Radiofrequency Radiation (RFR) emitted from both the cell phones and base stations have caused significant media attention and public discussion in recent years. The mechanism of interaction of RF fields with developing tissues of children and fetuses may be different from that of adults due to their smaller physical size and variation in tissue electromagnetic properties. The present study may provide an insight into the basic mechanisms by which RF fields interact with developing tissues in an embryo.
To evaluate the possible tissue and DNA damage in developing liver of chick embryo following chronic exposure to Ultra-High Frequency/Radiofrequency Radiation (UHF/RFR) emitted from 2G and 3G cell phone.
Fertilized chick embryos were incubated in four groups. Group A-experimental group exposed to 2G radiation (60 eggs), Group B- experimental group exposed to 3G radiation (60 eggs), Group C- sham exposed control group (60 eggs) and Group D- control group (48 eggs). On completion of scheduled duration, the embryos were collected and processed for routine histological studies to check structural changes in liver. The nuclear diameter and karyorrhexis changes of hepatocytes were analysed using oculometer and square reticule respectively. The liver procured from one batch of eggs from all the four groups was subjected to alkaline comet assay technique to assess DNA damage. The results were compared using one-way ANOVA test.
In our study, the exposure of developing chick embryos to 2G and 3G cell phone radiations caused structural changes in liver in the form of dilated sinusoidal spaces with haemorrhage, increased vacuolations in cytoplasm, increased nuclear diameter and karyorrhexis and significantly increased DNA damage.
The chronic exposure of chick embryo liver to RFR emitted from 2G and 3G cell phone resulted in various structural changes and DNA damage. The changes were more pronounced in 3G experimental group. Based on these findings it is necessary to create awareness among public about the possible ill effects of RFR exposure from cell phone.
近年来，手机和基站发出的射频辐射（RFR）对健康造成各种危害的科学证据不断增加，引起了媒体的广泛关注和公众讨论。由于儿童和胎儿的身体尺寸较小以及组织电磁特性的差异，射频场与他们发育中的组织的相互作用机制可能与成年人不同。本研究可能有助于深入了解射频场与胚胎发育中组织相互作用的基本机制。
评估慢性暴露于2G和3G手机发出的超高频/射频辐射（UHF/RFR）后，鸡胚发育中的肝脏可能出现的组织和DNA损伤。
将受精鸡胚分为四组进行孵化。A组为暴露于2G辐射的实验组（60枚蛋），B组为暴露于3G辐射的实验组（60枚蛋），C组为假暴露对照组（60枚蛋），D组为对照组（48枚蛋）。在预定时间结束后，收集胚胎并进行常规组织学研究，以检查肝脏的结构变化。分别使用目镜测微计和方格网分析肝细胞的核直径和核碎裂变化。从所有四组的一批蛋中获取的肝脏进行碱性彗星试验技术，以评估DNA损伤。使用单因素方差分析检验比较结果。
在我们的研究中，发育中的鸡胚暴露于2G和3G手机辐射会导致肝脏结构变化，表现为扩张的窦状隙伴出血、细胞质空泡化增加、核直径增大和核碎裂，以及DNA损伤显著增加。
鸡胚肝脏长期暴露于2G和3G手机发出的RFR会导致各种结构变化和DNA损伤。这些变化在3G实验组中更为明显。基于这些发现，有必要让公众了解手机RFR暴露可能产生的不良影响。
