Department of Urology, Diakonie Klinikum Stuttgart, Stuttgart, Germany.
World J Urol. 2013 Apr;31(2):351-8. doi: 10.1007/s00345-012-0925-y. Epub 2012 Aug 15.
Galactin-3 is a cell adhesion molecule involved in tumor progression. Our aim was to examine Gal-3 expression in tumor, benign tissue adjacent to the tumor (adjacent-benign) and benign prostate specimens and correlated it with biochemical recurrence.
Tissue microarrays were prepared from 83 tumor, 78 adjacent-benign and 75 benign tissues obtained from 83 patients undergoing prostatectomy for clinically localized prostate cancer. Tissues were stained using a Gal-3 antibody and immunohistochemistry. The staining was graded between 0 and 300 depending upon staining intensity and the area of staining. In 37 patients on whom there was follow-up (Mean: 57.8 months; Median: 68 months), staining intensity was correlated with biochemical recurrence.
Gal-3 showed both nuclear and cytoplasmic localization in benign, adjacent-benign and tumor tissues. Median Gal-3 staining scores significantly decreased from benign (192.5) to adjacent-benign (148.8 p = 0.007) and to tumor (108.8; p < 0.0001) tissues. In univariate analysis, age (p = 0.028), Gleason sum (p = 0.007), T stage (p = 0.011), seminal vesicle invasion (p = 0.009), pre-operative prostate-specific antigen (p = 0.045) and Gal-3 staining in tumor tissues (0.018) significantly correlated with biochemical recurrence. In multivariate analysis, Gal-3 expression in tumor (p = 0.04), adjacent-benign (p = 0.037) and benign (p = 0.005) tissues significantly correlated with biochemical recurrence. Gal-3 staining in tumor tissues had 91.7 % sensitivity, 64 % specificity and 73 % accuracy in predicting biochemical recurrence.
This is the first study that showed a decreasing gradient of Gal-3 expression in benign, adjacent-benign and tumor tissues. Gal-3 expression may be useful in predicting biochemical recurrence.
半乳糖凝集素-3 是一种参与肿瘤进展的细胞黏附分子。我们的目的是检测肿瘤、肿瘤旁良性组织（adjacent-benign）和良性前列腺组织中 Gal-3 的表达，并将其与生化复发相关联。
从 83 例接受前列腺切除术的局限性前列腺癌患者的 83 例肿瘤、78 例肿瘤旁良性组织和 75 例良性组织中制备组织微阵列。使用 Gal-3 抗体和免疫组织化学法对组织进行染色。根据染色强度和染色面积，将染色评分在 0 到 300 之间分级。在 37 例有随访的患者（平均：57.8 个月；中位数：68 个月）中，将染色强度与生化复发相关联。
Gal-3 在良性、肿瘤旁良性和肿瘤组织中均显示核和细胞质定位。中位 Gal-3 染色评分从良性组织（192.5）显著降低至肿瘤旁良性组织（148.8，p=0.007）和肿瘤组织（108.8；p<0.0001）。在单因素分析中，年龄（p=0.028）、Gleason 总和（p=0.007）、T 分期（p=0.011）、精囊侵犯（p=0.009）、术前前列腺特异性抗原（p=0.045）和肿瘤组织中的 Gal-3 染色（p=0.018）与生化复发显著相关。在多因素分析中，肿瘤（p=0.04）、肿瘤旁良性（p=0.037）和良性组织（p=0.005）中的 Gal-3 表达与生化复发显著相关。肿瘤组织中 Gal-3 染色的敏感性为 91.7%，特异性为 64%，准确性为 73%，预测生化复发的准确率较高。
这是第一项显示 Gal-3 在良性、肿瘤旁良性和肿瘤组织中表达逐渐降低的研究。Gal-3 的表达可能有助于预测生化复发。
