Department of Biology, Chair of Biochemistry, Faculty of Exact and Natural Sciences, Ivane Javakhishvili Tbilisi State University, Tbilisi, Georgia.
Department of Biology Chair of Immunology and Microbiology, Faculty of Exact and Natural Sciences, Ivane Javakhishvili Tbilisi State University, Tbilisi, Georgia.
Mol Biol Rep. 2022 Jan;49(1):443-450. doi: 10.1007/s11033-021-06894-w. Epub 2021 Nov 5.
Galectins-galactose-specific lectins are involved in various types of cell activities, including apoptosis, cell cycle regulation, inflammation and cell transformation. Galectins are implicated in prostate malignat transformation. It is not known yet if prostate glands with different grade of pathologies are expressing different galectins and if these galectins express different effects on the cell viability.
Cytosolic galactose-spesific lectin fractions from prostate tissue with different diagnosis were purified by affinity chromatography and analyzed by electrophoresis in polyacrylamide gel electrophoresis with sodium dodecyl sulphate. The lectin effects in a source-dependent maner were studied on cell viability on peripheral lymphocytes by MTT reduction method and on apoptosis by flow cytometry method.
Affinity purified galactose-specific lectins fractions from normal and pathological tissue samples are characterized with different protein composition and they express different effects on cell viability and apoptosis.
The effects of cytosolic galactose-specific lectins depend on the source of lectin fraction (glandular tissue disease). We suppose that the released cytosolic galectins from prostatic high grade intraepithelial neoplasia and adenocarcinoma tissue could suppress the immune status of the host patients.
半乳糖凝集素是一类特异性识别半乳糖的凝集素，参与细胞的多种活动，包括细胞凋亡、细胞周期调控、炎症和细胞转化。半乳糖凝集素与前列腺恶性转化有关。目前尚不清楚不同病变程度的前列腺是否表达不同的半乳糖凝集素，以及这些半乳糖凝集素对细胞活力是否有不同的影响。
通过亲和层析从不同诊断的前列腺组织中纯化细胞溶质半乳糖特异性凝集素级分，并通过聚丙烯酰胺凝胶电泳中的十二烷基硫酸钠电泳进行分析。通过 MTT 还原法研究了在源依赖性方式下，外周淋巴细胞的细胞活力和流式细胞术方法研究了细胞凋亡的凝集素作用。
从正常和病理组织样本中亲和纯化的半乳糖特异性凝集素级分具有不同的蛋白质组成，它们对半乳糖特异性凝集素对细胞活力和凋亡的影响也不同。
细胞溶质半乳糖特异性凝集素的作用取决于凝集素级分的来源（腺体组织疾病）。我们假设来自前列腺高级别上皮内瘤变和腺癌组织的释放的细胞溶质半乳糖凝集素可能会抑制宿主患者的免疫状态。
