Yuan Baohong, Burgess Sean A, Iranmahboob Amir, Bouchard Matthew B, Lehrer Nicole, Bordier Clémence, Hillman Elizabeth M C
Department of Biomedical Engineering, Laboratory for Functional Optical Imaging, Columbia University, New York 10027, USA.
Rev Sci Instrum. 2009 Apr;80(4):043706. doi: 10.1063/1.3117204.
Laminar optical tomography (LOT) is a new three-dimensional in vivo functional optical imaging technique. Adopting a microscopy-based setup and diffuse optical tomography (DOT) imaging principles, LOT can perform both absorption- and fluorescence-contrast imaging with higher resolution (100-200 microm) than DOT and deeper penetration (2-3 mm) than laser scanning microscopy. These features, as well as a large field of view and acquisition speeds up to 100 frames per second, make LOT suitable for depth-resolved imaging of stratified tissues such as retina, skin, endothelial tissues and the cortex of the brain. In this paper, we provide a detailed description of a new LOT system design capable of imaging both absorption and fluorescence contrast, and present characterization of its performance using phantom studies.
层流光学层析成像（LOT）是一种新型的三维活体功能光学成像技术。LOT采用基于显微镜的设置和扩散光学层析成像（DOT）原理，能够进行吸收对比成像和荧光对比成像，其分辨率（100 - 200微米）高于DOT，穿透深度（2 - 3毫米）比激光扫描显微镜更深。这些特性，以及大视野和高达每秒100帧的采集速度，使得LOT适用于对视网膜、皮肤、内皮组织和大脑皮层等分层组织进行深度分辨成像。在本文中，我们详细描述了一种能够同时进行吸收和荧光对比成像的新型LOT系统设计，并通过模型研究展示了其性能特征。
