MOE of Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
Biomaterials. 2013 Jan;34(4):975-84. doi: 10.1016/j.biomaterials.2012.10.041. Epub 2012 Nov 3.
The directional cell migration plays a crucial role in a variety of physiological and pathological processes. It can be controlled by the gradient cues immobilized on the substrate. The poly(sodium 4-styrenesulfonate) (PSS)/poly(diallyldimethylammonium) chloride (PDADMAC) multilayers were post-treated in a gradient NaCl solution with a concentration ranging from 3 M to 5 M, yielding the gradient multilayers with a similar chemistry composition (PSS domination) but gradually changed swelling ratio. The gradient nature and physicochemical properties were characterized by X-ray photoelectron spectroscopy and ellipsometry. Compared to the random migration with a lower rate at a smaller cell-seeding density, the vascular smooth muscle cells migrated directionally to the low hydration side at an appropriate cell-seeding density (1.5 × 10(4)/cm(2)) under the assistance of cell-cell interactions. The cell migration rates on the gradient surface were significantly larger than those on the corresponding uniform surfaces etched by salt solutions of the same concentrations. Relative cell adherent strength and focal adhesion formation were studied to unveil the intrinsic mechanism of the gradient multilayers on the cell migration. It was found that both the gradient cues and cell-cell contact have major influences on the directional cell migration.
定向细胞迁移在多种生理和病理过程中起着至关重要的作用。它可以通过固定在基底上的梯度线索来控制。聚（4-苯乙烯磺酸钠）（PSS）/聚（二烯丙基二甲基氯化铵）（PDADMAC）多层膜在浓度范围为 3 M 至 5 M 的梯度 NaCl 溶液中进行后处理，得到具有相似化学组成（PSS 占主导地位）但逐渐变化的溶胀比的梯度多层膜。通过 X 射线光电子能谱和椭圆光度法对梯度性质和物理化学性质进行了表征。与较低细胞接种密度下较低迁移率的随机迁移相比，在适当的细胞接种密度（1.5×10(4)/cm(2))下，在细胞-细胞相互作用的辅助下，血管平滑肌细胞定向迁移到低水合侧。在梯度表面上的细胞迁移速率明显大于用相同浓度的盐溶液刻蚀的相应均匀表面上的细胞迁移速率。研究了相对细胞黏附强度和焦点黏附形成，以揭示梯度多层膜对细胞迁移的内在机制。结果发现，梯度线索和细胞-细胞接触都对定向细胞迁移有重大影响。
