MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
Regen Biomater. 2014 Nov;1(1):17-25. doi: 10.1093/rb/rbu008. Epub 2014 Oct 20.
The surface-grafted poly(hydroxylethyl methacrylate) (PHEMA) molecules were demonstrated to show a brush state regardless of their molecular length (molecular weight). Adsorption of proteins from 10% fetal bovine serum (FBS), fibronectin (Fn) and bovine serum albumin (BSA) was quantified by ellipsometry, revealing that the amounts of FBS and Fn decreased monotonously along with the increase of PHEMA thickness, whereas not detectable for BSA when the PHEMA thickness was larger than 6 nm. Radio immunoassay found that the adsorption of Fn from 10% FBS had no significant difference regardless of the PHEMA thickness. However, ELISA results showed that the Arg-Gly-Asp (RGD) activity of adsorbed Fn decreased with the increase of PHEMA thickness. By comparison of cellular behaviors of vascular smooth muscle cells (VSMCs) being cultured in vitro in the normal serum-containing medium and the Fn-depleted serum-containing medium, the significant role of Fn on modulating the adhesion and migration of VSMCs was verified. Taking account all the results, the Fn adsorption model and its role on linking the biomaterials surface to the VSMCs behaviors are proposed.
表面接枝的聚羟乙基甲基丙烯酸酯（PHEMA）分子无论其分子长度（分子量）如何，均表现出刷状状态。通过椭圆测量法定量测定了来自 10%胎牛血清（FBS）、纤维连接蛋白（Fn）和牛血清白蛋白（BSA）的蛋白质的吸附，结果表明 FBS 和 Fn 的量随着 PHEMA 厚度的增加而单调减少，而当 PHEMA 厚度大于 6nm 时，BSA 则无法检测到。放射免疫测定发现，无论 PHEMA 厚度如何，来自 10%FBS 的 Fn 吸附均无显着差异。然而，ELISA 结果表明，吸附 Fn 的 Arg-Gly-Asp（RGD）活性随着 PHEMA 厚度的增加而降低。通过比较在含有正常血清的培养基中和 Fn 耗尽的血清培养基中体外培养的血管平滑肌细胞（VSMCs）的细胞行为，证实了 Fn 在调节 VSMCs 粘附和迁移中的重要作用。考虑到所有结果，提出了 Fn 吸附模型及其在将生物材料表面与 VSMCs 行为联系起来的作用。
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