Department of Ophthalmology, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan.
Theranostics. 2012;2(7):655-67. doi: 10.7150/thno.3890. Epub 2012 Jul 6.
Revealing the behavior of single molecules in living cells is very useful for understanding cellular events. Quantum dot probes are particularly promising tools for revealing how biological events occur at the single molecule level both in vitro and in vivo. In this review, we will introduce how single quantum dot applications are used for single molecule tracking. We will discuss how single quantum dot tracking has been used in several examples of complex biological processes, including membrane dynamics, neuronal function, selective transport mechanisms of the nuclear pore complex, and in vivo real-time observation. We also briefly discuss the prospects for single molecule tracking using advanced probes.
揭示活细胞中单分子的行为对于理解细胞事件非常有用。量子点探针是在体外和体内揭示生物事件如何在单分子水平上发生的极有前途的工具。在这篇综述中，我们将介绍如何将单量子点应用于单分子跟踪。我们将讨论单量子点跟踪在几个复杂生物过程中的应用，包括膜动力学、神经元功能、核孔复合体的选择性运输机制以及体内实时观察。我们还简要讨论了使用先进探针进行单分子跟踪的前景。
