Cardiology Department, University of Foggia, Viale Pinto 1, 71100, Foggia, Italy.
J Thromb Thrombolysis. 2012 Nov;34(4):475-82. doi: 10.1007/s11239-012-0761-3.
This study aimed to investigate lymphocyte populations in non-diabetic patients with early clinical presentation of coronary heart disease (CHD). Twenty-five consecutive middle-aged (<55 years) out-patients with CHD (acute myocardial infarction in the previous 3 months) and stable clinical conditions (>1 month) underwent venous blood sampling in order to determinate CD3+ (T-lymphocytes), CD19+ (B-lymphocytes), CD4+ (helper/inducer lymphocytes) and CD8+ (suppressor/cytotoxic lymphocytes) populations. Patients with diabetes, heart failure symptoms, recent revascularization (<30 days) were excluded. Twenty-five patients matched for age, gender and risk factors were enrolled as controls. All patients with CHD previously underwent coronary angiography. CHD patients showed lower CD3+ levels (70.96 ± 4.72 vs. 74.12 ± 4.93 %, p < 0.05) and CD8+ (37.80 ± 7.15 vs. 46.36 ± 5.22 %, p < 0.001) but higher CD4+ (37.32 ± 7.99 vs. 31.64 ± 4.72 %, p < 0.01) and CD4+/CD8+ ratio (1.06 ± 0.43 vs. 0.69 ± 0.13, p < 0.001). Difference in CD19+ levels was not statistically significant. Subjects with an impaired (≤55 %) left ventricular ejection fraction were characterized by lower levels of CD8+ (33.23 ± 7.04 vs. 43.76 ± 7.40 %, p < 0.05) and higher levels of CD4+ (38.31 ± 8.23 vs. 32.73 ± 6.08 %, p < 0.05) and CD4+/CD8+ ratio (1.06 ± 0.38 vs. 0.79 ± 0.34, p < 0.05). CD8+ levels inversely related to severity of coronary atherosclerosis (r = -0.37, p < 0.01). In conclusion, subjects with early clinical presentation of CHD are characterized by an altered CD4+/CD8+ ratio and lower CD3+ levels.
本研究旨在探讨非糖尿病患者早期冠心病（CHD）临床表型中的淋巴细胞群体。25 名连续的中年（<55 岁）门诊 CHD 患者（急性心肌梗死在过去 3 个月内）和稳定的临床状况（>1 个月）接受静脉采血，以确定 CD3+（T 淋巴细胞）、CD19+（B 淋巴细胞）、CD4+（辅助/诱导淋巴细胞）和 CD8+（抑制/细胞毒性淋巴细胞）群体。排除患有糖尿病、心力衰竭症状、近期血管重建术（<30 天）的患者。25 名年龄、性别和危险因素匹配的患者作为对照组。所有 CHD 患者均接受冠状动脉造影检查。CHD 患者的 CD3+水平（70.96 ± 4.72%比 74.12 ± 4.93%，p < 0.05）和 CD8+（37.80 ± 7.15%比 46.36 ± 5.22%，p < 0.001）降低，但 CD4+（37.32 ± 7.99%比 31.64 ± 4.72%，p < 0.01）和 CD4+/CD8+比值（1.06 ± 0.43 比 0.69 ± 0.13，p < 0.001）升高。CD19+水平的差异无统计学意义。左心室射血分数受损（≤55%）的患者其 CD8+水平较低（33.23 ± 7.04%比 43.76 ± 7.40%，p < 0.05），CD4+水平较高（38.31 ± 8.23%比 32.73 ± 6.08%，p < 0.05）和 CD4+/CD8+比值较高（1.06 ± 0.38 比 0.79 ± 0.34，p < 0.05）。CD8+水平与冠状动脉粥样硬化严重程度呈负相关（r = -0.37，p < 0.01）。总之，早期 CHD 临床表型的患者其 CD4+/CD8+比值和 CD3+水平发生改变。
