Garlichs C D, Cicha I, Raaz D, Meyer L, Stumpf C, Klinghammer L, Yilmaz A, Daniel W G
University of Erlangen-Nuremberg, Medical Clinic 2, Erlangen, Germany.
Inflamm Res. 2009 Jun;58(6):306-11. doi: 10.1007/s00011-008-8084-8.
Atherosclerosis, as an inflammatory disease, is characterized by pathologically altered levels of cytokines. We investigated whether smoking affects the CD40/CD154 system and pro-inflammatory cytokines in young males without other risk factors for atherosclerosis.
Young male smokers (n=13) and 14 non-smoking controls were investigated.
The differences in CD40/CD154 system and serum cytokines between the groups were measured using flow cytometry and ELISA.
In smokers, there was a strong trend (P<0.06) for increased CD40 expression on platelets as compared with non-smokers. However, there were no significant differences in CD40 expression on monocytes or in CD154 expression on platelets and T-cells between smokers and non-smokers. There was a strong trend for increased platelet-monocyte aggregates in smokers (P<0.06). Also, smokers had slightly but not significantly elevated hsCRP and IL-6 levels, and slightly decreased TNF-alpha and MCP-1. Interestingly, IL-18, a cytokine which has the ability to promote both Th1 and Th2 responses, was significantly decreased in smokers group (P=0.03 vs controls).
In young healthy males, smoking is not associated with dramatic changes in CD40/CD154 system. However, cigarette smoke alters the secreted cytokine profile, leading to significant decrease in systemic IL-18 levels.
动脉粥样硬化作为一种炎症性疾病，其特征在于细胞因子水平发生病理改变。我们研究了吸烟是否会影响无其他动脉粥样硬化危险因素的年轻男性的CD40/CD154系统和促炎细胞因子。
对年轻男性吸烟者（n = 13）和14名非吸烟对照者进行了研究。
使用流式细胞术和酶联免疫吸附测定法测量两组之间CD40/CD154系统和血清细胞因子的差异。
与非吸烟者相比，吸烟者血小板上CD40表达有强烈升高趋势（P < 0.06）。然而，吸烟者与非吸烟者之间单核细胞上CD40表达以及血小板和T细胞上CD154表达无显著差异。吸烟者中血小板 - 单核细胞聚集体有强烈增加趋势（P < 0.06）。此外，吸烟者的hsCRP和IL - 6水平略有升高但无统计学意义，TNF - α和MCP - 1略有下降。有趣的是，具有促进Th1和Th2反应能力的细胞因子IL - 18在吸烟者组中显著降低（与对照组相比，P = 0.03）。
在年轻健康男性中，吸烟与CD40/CD154系统的显著变化无关。然而，香烟烟雾会改变分泌的细胞因子谱，导致全身IL - 18水平显著降低。
