Departamento de Química, Universidad Nacional de Colombia, AA 14490, Bogotá, Colombia.
J Nat Prod. 2012 Sep 28;75(9):1637-42. doi: 10.1021/np300313k. Epub 2012 Aug 20.
Three new cembranoid diterpenes, knightine (1), 11(R)-hydroxy-12(20)-en-knightal (2), and 11(R)-hydroxy-12(20)-en-knightol acetate (3), were isolated as minor constituents of the Caribbean gorgonian Eunicea knighti, along with the known cembranoids 4-8. The stereostructures of the new compounds were determined by detailed spectroscopic analyses and a combination of chemical transformations and modified Mosher's methods. All isolated cembranoids were tested against fouling using a quorum-sensing inhibition (QSI) assay and a biofilm inhibition test. Compounds 2, 3, and 6 disrupted QS systems at lower concentrations than kojic acid and Cu(2)O, and in most cases cembranoids 1-8 showed bacterial biofilm inhibition at lower concentrations than kojic acid.
三种新的海鞘烷二萜类化合物，knightine（1）、11(R)-羟基-12(20)-烯-knightal（2）和 11(R)-羟基-12(20)-烯-knightol 乙酸酯（3），作为加勒比海柳珊瑚 Eunicea knighti 的次要成分被分离出来，同时还分离出了已知的海鞘烷类化合物 4-8。新化合物的立体结构通过详细的光谱分析以及化学转化和改良的 Mosher 方法的组合来确定。所有分离出的海鞘烷类化合物都通过群体感应抑制（QSI）测定和生物膜抑制试验进行了抗污损测试。化合物 2、3 和 6 在较低浓度下就能破坏 QS 系统，比 kojic 酸和 Cu(2)O 的效果更好，而且在大多数情况下，海鞘烷类化合物 1-8 在较低浓度下就能抑制细菌生物膜的形成，优于 kojic 酸。
