Yu J, Fei J, Azad J, Gong M, Lan Y, Chen G
Department of Surgery, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
J Int Med Res. 2012;40(3):1016-24. doi: 10.1177/147323001204000320.
To investigate the myocardially protective effects of Salvia miltiorrhiza injection in streptozotocin-induced diabetic rats and the possible mechanisms involved.
Adult male Sprague-Dawley rats were randomized into three groups (n = 10 per group): diabetes, no treatment (Sm-); diabetes, S. miltiorrhiza injection (Sm+); control (no diabetes; saline treatment). After model induction and 4 weeks' treatment, heart function of five rats from each group was tested by Langendorff isolated in vivo heart perfusion. In the remaining rats, pathological changes of the myocardium were observed by haematoxylin and eosin staining, and protein levels of thrombospondin-1 (TSP-1) and transforming growth factor-β1 (TGF-β1) were assessed by immunohistochemistry.
Left ventricular systolic end pressure and left ventricular developed pressure were significantly improved in the Sm+ group compared with the Sm- group. Pathological changes were ameliorated through significantly reduced TSP-1 and TGF-β1 protein levels.
S. miltiorrhiza injection may improve the heart function of diabetic rats and protect against cardiomyopathy by downregulating TSP-1 and TGF-β1 in myocardial tissue.
探讨丹参注射液对链脲佐菌素诱导的糖尿病大鼠的心肌保护作用及其可能机制。
成年雄性Sprague-Dawley大鼠随机分为三组（每组n = 10）：糖尿病未治疗组（Sm-）；糖尿病丹参注射液治疗组（Sm+）；对照组（非糖尿病；生理盐水治疗）。模型诱导及4周治疗后，每组取5只大鼠通过Langendorff离体心脏灌注检测心功能。其余大鼠通过苏木精-伊红染色观察心肌病理变化，免疫组化法检测血小板反应蛋白-1（TSP-1）和转化生长因子-β1（TGF-β1）蛋白水平。
与Sm-组相比，Sm+组左心室收缩末期压力和左心室舒张末期压力显著改善。通过显著降低TSP-1和TGF-β1蛋白水平改善了病理变化。
丹参注射液可能通过下调心肌组织中TSP-1和TGF-β1来改善糖尿病大鼠的心功能并预防心肌病。
