Department of Ophthalmology, Buenos Aires University, Buenos Aires, Argentina.
Ophthalmic Res. 2012;48 Suppl 1:21-5. doi: 10.1159/000339845. Epub 2012 Aug 21.
Brilliant blue G (BBG) is frequently used in chromovitrectomy to facilitate internal limiting membrane (ILM) peeling. A study was initiated to evaluate if heavy BBG is safe and effective in staining the ILM.
We studied 30 eyes, 23 with idiopathic macular holes and 7 of patients with diabetic macular edema. Removal of the ILMs was assisted by heavy BBG staining. In cases with histopathological correlation the ILMs were evaluated with hematoxylin and eosin, Masson's trichrome, periodic acid-Schiff and glial fibrillary acidic protein staining. In addition, immunohistochemistry was also performed using specific antibodies for vimentin, neuron-specific enolase, factor VIII and CD68. Using the Image-Pro Plus software of Media Cybernetics Co. we found an average thickness in ILMs.
Of the ILM specimens sent, 19/30 (63.33%) could not be processed properly because of the limited sample material, recognizing only fragments of dispersed fibrillar material. In macular hole ILMs we found an average thickness of 1.3 ± 0.65 µm, and in diabetic macular edema ILMs an average thickness of 6.2 ± 1.4 µm.
In heavy BBG-assisted ILM peeling we observed no intraoperative or postoperative complications after a mean follow-up of 12 months. Heavy BBG could be an effective and safe vehicle for staining the ILM.
亮蓝 G（BBG）常用于彩色玻璃体切除术，以辅助内界膜（ILM）剥离。本研究旨在评估重 BBG 染色 ILM 是否安全有效。
我们研究了 30 只眼，23 只为特发性黄斑裂孔，7 只为糖尿病性黄斑水肿患者。通过重 BBG 染色辅助去除 ILM。有组织病理学相关性的病例，采用苏木精和伊红、马松三色、过碘酸-Schiff 和胶质纤维酸性蛋白染色对 ILM 进行评估。此外，还使用针对波形蛋白、神经元特异性烯醇化酶、因子 VIII 和 CD68 的特异性抗体进行免疫组织化学染色。使用 Media Cybernetics Co. 的 Image-Pro Plus 软件，我们发现 ILM 的平均厚度为 1.3±0.65µm。在黄斑裂孔的 ILM 中，我们发现平均厚度为 1.3±0.65µm，而在糖尿病性黄斑水肿的 ILM 中，平均厚度为 6.2±1.4µm。
在重 BBG 辅助的 ILM 剥离中，我们观察到平均 12 个月的随访中没有术中或术后并发症。重 BBG 可能是一种有效且安全的 ILM 染色剂。
