Department of Pediatrics, Baylor College of Medicine, Houston, Texas, USA.
Pediatr Res. 2012 Nov;72(5):502-6. doi: 10.1038/pr.2012.115. Epub 2012 Aug 20.
Ureaplasma causes sepsis in human neonates. Although erythromycin has been the standard treatment, it is not always effective. No published reports have evaluated Ureaplasma sepsis in a neonatal model. We hypothesized that appropriate antibiotic treatment improves Ureaplasma sepsis in a neonatal mouse model.
Two ATCC strains and two clinical strains of Ureaplasma were evaluated in vitro for antibiotic minimum inhibitory concentration (MIC). In addition, FVB albino mice pups infected with Ureaplasma were randomly assigned to saline, erythromycin, or azithromycin therapy and survival, quantitative blood culture, and growth were evaluated.
MICs ranged from 0.125 to 62.5 µg/ml and 0.25 to 1.0 µg/ml for erythromycin and azithromycin, respectively. The infecting strain and antibiotic selected for treatment appeared to affect survival and bacteremia, but only the infecting strain affected growth. Azithromycin improved survival and bacteremia against each strain, whereas erythromycin was effective against only one of four strains.
We have established a neonatal model of Ureaplasma sepsis and observed that treatment outcome is related to infecting strain and antibiotic treatment. We speculate that appropriate antibiotic selection and dosing are required for effective treatment of Ureaplasma sepsis in neonates, and this model could be used to further evaluate these relationships.
脲原体可导致人类新生儿败血症。尽管红霉素一直是标准治疗方法，但并不总是有效。尚无文献报道评估过新生儿模型中的脲原体败血症。我们假设适当的抗生素治疗可改善新生儿脲原体败血症模型中的脲原体败血症。
我们评估了 ATCC 株和两株临床分离株脲原体的体外抗生素最小抑菌浓度（MIC）。此外，用脲原体感染 FVB 白化鼠幼仔，然后将其随机分为盐水、红霉素或阿奇霉素治疗组，并评估其存活率、定量血培养和生长情况。
红霉素和阿奇霉素的 MIC 值分别为 0.125 至 62.5μg/ml 和 0.25 至 1.0μg/ml。感染株和所选抗生素似乎影响存活率和菌血症，但只有感染株影响生长。阿奇霉素改善了对每种菌株的存活率和菌血症，而红霉素仅对四种菌株中的一种有效。
我们建立了新生儿脲原体败血症模型，并观察到治疗结果与感染株和抗生素治疗有关。我们推测，新生儿脲原体败血症的有效治疗需要选择合适的抗生素并进行适当的剂量调整，该模型可用于进一步评估这些关系。
