A 类碳青霉烯酶 KPC-6 的耐药性和底物谱。
Antibiotic resistance and substrate profiles of the class A carbapenemase KPC-6.
机构信息
Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, Indiana, USA.
出版信息
Antimicrob Agents Chemother. 2012 Nov;56(11):6006-8. doi: 10.1128/AAC.01338-12. Epub 2012 Aug 20.
Abstract
The class A carbapenemase KPC-6 produces resistance to a broad range of β-lactam antibiotics. This enzyme hydrolyzes penicillins, the monobactam aztreonam, and carbapenems with similar catalytic efficiencies, ranging from 10(5) to 10(6) M(-1) s(-1). The catalytic efficiencies of KPC-6 against cephems vary to a greater extent, ranging from 10(3) M(-1) s(-1) for the cephamycin cefoxitin and the extended-spectrum cephalosporin ceftazidime to 10(5) to 10(6) M(-1) s(-1) for the narrow-spectrum and some of the extended-spectrum cephalosporins.
摘要
A 类碳青霉烯酶 KPC-6 可导致对多种β-内酰胺类抗生素产生耐药性。该酶对青霉素类、单环β-内酰胺类氨曲南和碳青霉烯类的水解速度相似，酶动力学常数（Kcat）在 10(5) 到 10(6) M(-1) s(-1) 之间。KPC-6 对头孢菌素类的催化效率差异较大，头孢噻肟的 Kcat 值为 10(3) M(-1) s(-1)，头孢西丁为头孢菌素类的碳青霉烯类，而头孢他啶和头孢吡肟的 Kcat 值为 10(5) 到 10(6) M(-1) s(-1)。
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