• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Rab10 和肌球蛋白 Va 介导脂肪细胞中胰岛素刺激的 GLUT4 储存囊泡转运。

Rab10 and myosin-Va mediate insulin-stimulated GLUT4 storage vesicle translocation in adipocytes.

机构信息

Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.

出版信息

J Cell Biol. 2012 Aug 20;198(4):545-60. doi: 10.1083/jcb.201111091.

DOI:10.1083/jcb.201111091
PMID:22908308
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3514028/
Abstract

Rab proteins are important regulators of insulin-stimulated GLUT4 translocation to the plasma membrane (PM), but the precise steps in GLUT4 trafficking modulated by particular Rab proteins remain unclear. Here, we systematically investigate the involvement of Rab proteins in GLUT4 trafficking, focusing on Rab proteins directly mediating GLUT4 storage vesicle (GSV) delivery to the PM. Using dual-color total internal reflection fluorescence (TIRF) microscopy and an insulin-responsive aminopeptidase (IRAP)-pHluorin fusion assay, we demonstrated that Rab10 directly facilitated GSV translocation to and docking at the PM. Rab14 mediated GLUT4 delivery to the PM via endosomal compartments containing transferrin receptor (TfR), whereas Rab4A, Rab4B, and Rab8A recycled GLUT4 through the endosomal system. Myosin-Va associated with GSVs by interacting with Rab10, positioning peripherally recruited GSVs for ultimate fusion. Thus, multiple Rab proteins regulate the trafficking of GLUT4, with Rab10 coordinating with myosin-Va to mediate the final steps of insulin-stimulated GSV translocation to the PM.

摘要

Rab 蛋白是胰岛素刺激 GLUT4 向质膜（PM）易位的重要调节剂，但特定 Rab 蛋白调节 GLUT4 运输的精确步骤仍不清楚。在这里，我们系统地研究了 Rab 蛋白在 GLUT4 运输中的作用，重点研究了直接介导 GLUT4 储存囊泡（GSV）向 PM 传递的 Rab 蛋白。我们使用双色全内反射荧光（TIRF）显微镜和胰岛素反应性氨肽酶（IRAP）-pHluorin 融合测定法，证明 Rab10 直接促进 GSV 向 PM 的易位和停靠。Rab14 通过含有转铁蛋白受体（TfR）的内体区室介导 GLUT4 向 PM 的传递，而 Rab4A、Rab4B 和 Rab8A 通过内体系统回收 GLUT4。肌球蛋白-Va 通过与 Rab10 相互作用与 GSV 相关联，将外周募集的 GSV 定位用于最终融合。因此，多种 Rab 蛋白调节 GLUT4 的运输，Rab10 与肌球蛋白-Va 协调介导胰岛素刺激的 GSV 向 PM 易位的最后步骤。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1289/3514028/2752d87882cd/JCB_201111091_Fig10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1289/3514028/74f618b20848/JCB_201111091_Fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1289/3514028/10da11f5d657/JCB_201111091_Fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1289/3514028/cc25dd7fc2ff/JCB_201111091_Fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1289/3514028/87b0861033f6/JCB_201111091_Fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1289/3514028/6a8098497efd/JCB_201111091_Fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1289/3514028/34f11ce52ae1/JCB_201111091_Fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1289/3514028/de6e2a3a70cb/JCB_201111091_Fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1289/3514028/2cb4a645e959/JCB_201111091_Fig8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1289/3514028/e2a25513edbd/JCB_201111091_Fig9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1289/3514028/2752d87882cd/JCB_201111091_Fig10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1289/3514028/74f618b20848/JCB_201111091_Fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1289/3514028/10da11f5d657/JCB_201111091_Fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1289/3514028/cc25dd7fc2ff/JCB_201111091_Fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1289/3514028/87b0861033f6/JCB_201111091_Fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1289/3514028/6a8098497efd/JCB_201111091_Fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1289/3514028/34f11ce52ae1/JCB_201111091_Fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1289/3514028/de6e2a3a70cb/JCB_201111091_Fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1289/3514028/2cb4a645e959/JCB_201111091_Fig8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1289/3514028/e2a25513edbd/JCB_201111091_Fig9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1289/3514028/2752d87882cd/JCB_201111091_Fig10.jpg

相似文献

1
Rab10 and myosin-Va mediate insulin-stimulated GLUT4 storage vesicle translocation in adipocytes.Rab10 和肌球蛋白 Va 介导脂肪细胞中胰岛素刺激的 GLUT4 储存囊泡转运。
J Cell Biol. 2012 Aug 20;198(4):545-60. doi: 10.1083/jcb.201111091.
2
Rab10 delivers GLUT4 storage vesicles to the plasma membrane.Rab10将葡萄糖转运蛋白4储存囊泡转运至质膜。
Commun Integr Biol. 2013 May 1;6(3):e23779. doi: 10.4161/cib.23779. Epub 2013 May 13.
3
Myosin IIA participates in docking of Glut4 storage vesicles with the plasma membrane in 3T3-L1 adipocytes.肌球蛋白 IIA 参与了 3T3-L1 脂肪细胞中 Glut4 储存小泡与质膜的对接。
Biochem Biophys Res Commun. 2010 Jan 1;391(1):995-9. doi: 10.1016/j.bbrc.2009.12.004. Epub 2009 Dec 5.
4
Rab10, a target of the AS160 Rab GAP, is required for insulin-stimulated translocation of GLUT4 to the adipocyte plasma membrane.Rab10是AS160 Rab GAP的一个靶点，胰岛素刺激葡萄糖转运蛋白4（GLUT4）转位至脂肪细胞质膜需要Rab10。
Cell Metab. 2007 Apr;5(4):293-303. doi: 10.1016/j.cmet.2007.03.001.
5
Myosin Va mediates Rab8A-regulated GLUT4 vesicle exocytosis in insulin-stimulated muscle cells.肌球蛋白Va介导胰岛素刺激的肌肉细胞中Rab8A调节的葡萄糖转运蛋白4囊泡胞吐作用。
Mol Biol Cell. 2014 Apr;25(7):1159-70. doi: 10.1091/mbc.E13-08-0493. Epub 2014 Jan 29.
6
Rab14 limits the sorting of Glut4 from endosomes into insulin-sensitive regulated secretory compartments in adipocytes.Rab14限制了脂肪细胞中Glut4从内体向胰岛素敏感的调节性分泌小室的分选。
Biochem J. 2016 May 15;473(10):1315-27. doi: 10.1042/BCJ20160020. Epub 2016 Mar 2.
7
SEC16A is a RAB10 effector required for insulin-stimulated GLUT4 trafficking in adipocytes.SEC16A是脂肪细胞中胰岛素刺激的GLUT4转运所需的一种RAB10效应蛋白。
J Cell Biol. 2016 Jul 4;214(1):61-76. doi: 10.1083/jcb.201509052. Epub 2016 Jun 27.
8
Insulin triggers surface-directed trafficking of sequestered GLUT4 storage vesicles marked by Rab10.胰岛素触发由Rab10标记的隔离GLUT4储存囊泡的表面定向运输。
Small GTPases. 2013 Jul-Sep;4(3):193-7. doi: 10.4161/sgtp.26471. Epub 2013 Sep 12.
9
A role for Rab14 in the endocytic trafficking of GLUT4 in 3T3-L1 adipocytes.Rab14 在 3T3-L1 脂肪细胞中 GLUT4 的内吞运输中的作用。
J Cell Sci. 2013 May 1;126(Pt 9):1931-41. doi: 10.1242/jcs.104307. Epub 2013 Feb 26.
10
Rab10 in insulin-stimulated GLUT4 translocation.Rab10在胰岛素刺激的葡萄糖转运蛋白4转位过程中的作用
Biochem J. 2008 Apr 1;411(1):89-95. doi: 10.1042/BJ20071318.

引用本文的文献

1
GLUT4 Trafficking and Storage Vesicles: Molecular Architecture, Regulatory Networks, and Their Disruption in Insulin Resistance.葡萄糖转运蛋白4（GLUT4）的运输与储存囊泡：分子结构、调控网络及其在胰岛素抵抗中的破坏
Int J Mol Sci. 2025 Aug 5;26(15):7568. doi: 10.3390/ijms26157568.
2
RAB-10 cooperates with EHBP-1 to capture vesicular carriers during post-Golgi exocytic trafficking.RAB-10与EHBP-1协同作用，在高尔基体后胞吐运输过程中捕获囊泡载体。
J Cell Biol. 2025 Apr 7;224(4). doi: 10.1083/jcb.202410003. Epub 2025 Feb 21.
3
Endosomal catabolism of phosphatidylinositol 4,5-bisphosphate is fundamental in building resilience against pathogens.

本文引用的文献

1
Dual-mode of insulin action controls GLUT4 vesicle exocytosis.胰岛素作用的双重模式控制 GLUT4 囊泡胞吐作用。
J Cell Biol. 2011 May 16;193(4):643-53. doi: 10.1083/jcb.201008135. Epub 2011 May 9.
2
Insulin-stimulated GLUT4 protein translocation in adipocytes requires the Rab10 guanine nucleotide exchange factor Dennd4C.胰岛素刺激脂肪细胞中 GLUT4 蛋白易位需要 Rab10 鸟嘌呤核苷酸交换因子 Dennd4C。
J Biol Chem. 2011 May 13;286(19):16541-5. doi: 10.1074/jbc.C111.228908. Epub 2011 Mar 22.
3
Endocytosis, recycling, and regulated exocytosis of glucose transporter 4.
磷脂酰肌醇4,5-二磷酸的内体分解代谢对于建立抵御病原体的能力至关重要。
Protein Cell. 2025 Mar 8;16(3):161-187. doi: 10.1093/procel/pwae041.
4
Rab10-CAV1 mediated intraluminal vesicle transport to migrasomes.Rab10-CAV1 介导的管腔内囊泡转运至迁移体。
Proc Natl Acad Sci U S A. 2024 Jul 23;121(30):e2319267121. doi: 10.1073/pnas.2319267121. Epub 2024 Jul 15.
5
Exercise training and cold exposure trigger distinct molecular adaptations to inguinal white adipose tissue.运动训练和冷暴露会引发腹股沟白色脂肪组织的明显分子适应。
Cell Rep. 2024 Jul 23;43(7):114481. doi: 10.1016/j.celrep.2024.114481. Epub 2024 Jul 13.
6
Research Advances in Fusion Protein-Based Drugs for Diabetes Treatment.基于融合蛋白的糖尿病治疗药物研究进展
Diabetes Metab Syndr Obes. 2024 Jan 23;17:343-362. doi: 10.2147/DMSO.S421527. eCollection 2024.
7
Monoamine oxidase mediated oxidative stress: a potential molecular and biochemical crux in the pathogenesis of obesity.单胺氧化酶介导的氧化应激：肥胖发病机制中的潜在分子和生化关键。
Mol Biol Rep. 2023 Dec 23;51(1):29. doi: 10.1007/s11033-023-08938-9.
8
Akt may associate with insulin-responsive vesicles via interaction with sortilin.Akt 可能通过与分选连接蛋白的相互作用与胰岛素反应性囊泡结合。
FEBS Lett. 2024 Feb;598(4):390-399. doi: 10.1002/1873-3468.14790. Epub 2023 Dec 21.
9
Regulation of De Novo Lipid Synthesis by the Small GTPase Rac1 in the Adipogenic Differentiation of Progenitor Cells from Mouse White Adipose Tissue.小 GTP 酶 Rac1 在小鼠白色脂肪组织祖细胞成脂分化中对从头合成脂质的调控作用。
Int J Mol Sci. 2023 Feb 27;24(5):4608. doi: 10.3390/ijms24054608.
10
A high-content endogenous GLUT4 trafficking assay reveals new aspects of adipocyte biology.高内涵内源性 GLUT4 转运分析揭示了脂肪细胞生物学的新方面。
Life Sci Alliance. 2022 Oct 25;6(1). doi: 10.26508/lsa.202201585. Print 2023 Jan.
葡萄糖转运蛋白 4 的内吞作用、回收和受调控的胞吐作用。
Biochemistry. 2011 Apr 19;50(15):3048-61. doi: 10.1021/bi2000356. Epub 2011 Mar 25.
4
Role of Rab GTPases in membrane traffic and cell physiology.Rab GTPases 在膜转运和细胞生理学中的作用。
Physiol Rev. 2011 Jan;91(1):119-49. doi: 10.1152/physrev.00059.2009.
5
Rab8A and Rab13 are activated by insulin and regulate GLUT4 translocation in muscle cells.Rab8A 和 Rab13 被胰岛素激活，并调节肌肉细胞中的 GLUT4 易位。
Proc Natl Acad Sci U S A. 2010 Nov 16;107(46):19909-14. doi: 10.1073/pnas.1009523107. Epub 2010 Nov 1.
6
Identification of a distal GLUT4 trafficking event controlled by actin polymerization.鉴定由肌动蛋白聚合作用控制的GLUT4远端转运事件。
Mol Biol Cell. 2009 Sep;20(17):3918-29. doi: 10.1091/mbc.e09-03-0187. Epub 2009 Jul 15.
7
Rab GTPases as coordinators of vesicle traffic.作为囊泡运输协调因子的Rab小GTP酶
Nat Rev Mol Cell Biol. 2009 Aug;10(8):513-25. doi: 10.1038/nrm2728. Epub 2009 Jul 15.
8
Rab4b is a small GTPase involved in the control of the glucose transporter GLUT4 localization in adipocyte.Rab4b是一种小GTP酶，参与控制脂肪细胞中葡萄糖转运蛋白GLUT4的定位。
PLoS One. 2009 Apr 17;4(4):e5257. doi: 10.1371/journal.pone.0005257.
9
Dual role for myosin II in GLUT4-mediated glucose uptake in 3T3-L1 adipocytes.肌球蛋白II在3T3-L1脂肪细胞中GLUT4介导的葡萄糖摄取中的双重作用。
Exp Cell Res. 2008 Oct 15;314(17):3264-74. doi: 10.1016/j.yexcr.2008.08.007. Epub 2008 Aug 22.
10
Emerging role for AS160/TBC1D4 and TBC1D1 in the regulation of GLUT4 traffic.AS160/TBC1D4和TBC1D1在调节GLUT4转运中的新作用。
Am J Physiol Endocrinol Metab. 2008 Jul;295(1):E29-37. doi: 10.1152/ajpendo.90331.2008. Epub 2008 May 13.