Jitvaropas Rungrat, Saenthaweesuk Suphaket, Somparn Nuntiya, Thuppia Amornnat, Sireeratawong Seewaboon, Phoolcharoen Waranyoo
Department of Preclinical Science, Faculty of Medicine, Thammasat University, Rangsit Campus, Khlong Luang, Pathum Thani, 12120 Thailand.
Nat Prod Commun. 2012 Jul;7(7):909-12.
The ethanolic extract of Boesenbergia rotunda (L.) Mansf was studied for its wound-healing potential. Since wound healing is interrelated with microbial infection and reactive oxygen species (ROS), this study was conducted to evaluate the antimicrobial and antioxidant activity of B. rotunda. The antimicrobial activity of B. rotunda was studied against six bacterial and two yeast strains using disc diffusion, minimum inhibitory concentration (MIC), and minimum microbicidal concentration (MMC). The B. rotunda extract displayed potential antimicrobial and antifungal activities by inhibiting the Gram-positive bacteria Staphylococcus aureus (ATCC 25923), S. epidermidis, and Bacillus subtilis (ATCC 6633), and the yeasts Candida albicans (ATCC 10231), and Saccharomyces cerevisiae. MIC and MMC values varied from 0.04 to 25 mg/mL and from 0.16 to 25 mg/mL, respectively. The antioxidant activity of B. rotunda was evaluated by measuring the Ferric Reducing/Antioxidant Power (FRAP) and DPPH free radical scavenging activity. The FRAP and DPPH values were 22.2 microM/microg and 76.3 mg/mL, respectively. In the wound-healing studies, the topical application of the B. rotunda extract indicated a significantly increased percentage of wound contraction on day 12 compared with the control group. Histological studies showed the complete epidermis and found collagen fibers and hair follicles in the dermis. The results of the present study support the continued and expanded utilization of B. rotunda in Thai folk medicine.
对圆叶山姜（Boesenbergia rotunda (L.) Mansf）的乙醇提取物进行了伤口愈合潜力研究。由于伤口愈合与微生物感染和活性氧（ROS）相关，因此开展本研究以评估圆叶山姜的抗菌和抗氧化活性。采用纸片扩散法、最低抑菌浓度（MIC）和最低杀菌浓度（MMC）研究了圆叶山姜对六种细菌菌株和两种酵母菌株的抗菌活性。圆叶山姜提取物通过抑制革兰氏阳性菌金黄色葡萄球菌（ATCC 25923）、表皮葡萄球菌、枯草芽孢杆菌（ATCC 6633）以及酵母白色念珠菌（ATCC 10231）和酿酒酵母，显示出潜在的抗菌和抗真菌活性。MIC和MMC值分别在0.04至25 mg/mL和0.16至25 mg/mL之间变化。通过测量铁还原/抗氧化能力（FRAP）和DPPH自由基清除活性评估了圆叶山姜的抗氧化活性。FRAP和DPPH值分别为22.2 microM/μg和76.3 mg/mL。在伤口愈合研究中，与对照组相比，圆叶山姜提取物局部应用在第12天显示伤口收缩百分比显著增加。组织学研究显示表皮完整，真皮中有胶原纤维和毛囊。本研究结果支持在泰国传统医学中持续且扩大使用圆叶山姜。
