TU Braunschweig, Germany.
Lab Chip. 2014 Oct 21;14(20):4042-9. doi: 10.1039/c4lc00786g.
Currently, the Lateral flow Immunoassays (LFIAs) are not able to perform complex multi-step immunodetection tests because of their inability to introduce multiple reagents in a controlled manner to the detection area autonomously. In this research, a point-of-care (POC) paper-based lateral flow immunosensor was developed incorporating a novel microfluidic valve technology. Layers of paper and tape were used to create a three-dimensional structure to form the fluidic network. Unlike the existing LFIAs, multiple directional valves are embedded in the test strip layers to control the order and the timing of mixing for the sample and multiple reagents. In this paper, we report a four-valve device which autonomously directs three different fluids to flow sequentially over the detection area. As proof of concept, a three-step alkaline phosphatase based Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol with Rabbit IgG as the model analyte was conducted to prove the suitability of the device for immunoassays. The detection limit of about 4.8 fm was obtained.
目前，由于侧向流动免疫分析（LFIAs）无法自主地将多种试剂以受控的方式引入检测区域，因此无法进行复杂的多步免疫检测测试。在这项研究中，开发了一种即时（POC）基于纸张的侧向流动免疫传感器，该传感器结合了新颖的微流控阀技术。使用纸和胶带的层创建三维结构以形成流体网络。与现有的 LFIAs 不同，多个定向阀嵌入测试条层中以控制样品和多种试剂的混合顺序和时间。在本文中，我们报告了一种四阀装置，该装置可自动将三种不同的流体顺序引导至检测区域。作为概念验证，进行了基于碱性磷酸酶的三步酶联免疫吸附测定（ELISA）方案，以兔 IgG 作为模型分析物，证明了该设备适用于免疫测定。获得了约 4.8 fm 的检测限。
