Draper Bruce W
Department of Molecular and Cellular Biology, University of California Davis, Davis, CA, USA.
Methods Mol Biol. 2012;916:157-65. doi: 10.1007/978-1-61779-980-8_12.
Zebrafish breed year round and females are capable of producing thousands of eggs during their lifetime. This amazing fecundity is due to the fact that the adult ovary, contains premeiotic oocyte progenitor cells, called oogonia, which produce a continuous supply of new oocytes throughout adult life. Oocyte progenitor cells can be easily identified based on their expression of Vasa, and their characteristic nuclear morphology. Thus, the zebrafish ovary provides a unique and powerful system to study the genetic regulation of oocyte production in a vertebrate animal. A method is presented here for identifying oocyte progenitor cells in the zebrafish ovary using whole-mount confocal immunofluorescence that is simple and accurate.
斑马鱼全年均可繁殖，雌性斑马鱼一生能够产出数千枚卵。这种惊人的繁殖力归因于成年卵巢中含有减数分裂前的卵母细胞祖细胞，即卵原细胞，它们在成年期持续产生新的卵母细胞。卵母细胞祖细胞可根据其Vasa表达和特征性核形态轻松识别。因此，斑马鱼卵巢为研究脊椎动物中卵母细胞生成的遗传调控提供了一个独特而强大的系统。本文介绍了一种使用全组织共聚焦免疫荧光技术在斑马鱼卵巢中识别卵母细胞祖细胞的方法，该方法简单且准确。
