使用针对erm rRNA甲基化酶基因保守区域的引物通过聚合酶链反应检测红霉素耐药性。
Detection of erythromycin resistance by the polymerase chain reaction using primers in conserved regions of erm rRNA methylase genes.
作者信息
Arthur M, Molinas C, Mabilat C, Courvalin P
机构信息
Unité des Agents Antibactériens, Institut Pasteur, Paris, France.
出版信息
Antimicrob Agents Chemother. 1990 Oct;34(10):2024-6. doi: 10.1128/AAC.34.10.2024.
Abstract
Genes belonging to different erm DNA hybridization classes were selectively amplified by polymerase chain reaction with a pair of oligonucleotides that corresponded to conserved amino acid motifs in known ERM methylases. Identification of the resistance mechanism was possible despite substantial nucleotide sequence diversity among the erythromycin resistance genes.
摘要
通过聚合酶链反应，使用一对与已知ERM甲基化酶中保守氨基酸基序相对应的寡核苷酸，对属于不同erm DNA杂交类别的基因进行选择性扩增。尽管红霉素抗性基因之间存在大量核苷酸序列差异，但仍有可能鉴定出抗性机制。
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