Department of Animal Science and Institute of Rare Earth for Biological Application, Chonbuk National University, Jeonju 561-756, Republic of Korea.
Appl Microbiol Biotechnol. 2013 Feb;97(4):1725-34. doi: 10.1007/s00253-012-4353-0. Epub 2012 Aug 24.
Zinc oxide (ZnO) nanostructures have been commonly studied for electronic purposes due to their unique piezoelectric and catalytic properties; however, recently, they have been also exploited for biomedical applications. The purpose of this study was to fabricate ZnO-doped poly(urethane) (PU) nanocomposite via one-step electrospinning technique. The utilized nanocomposite was prepared by using colloidal gel composed of ZnO and PU, and the obtained mats were vacuum dried at 60 °C overnight. The physicochemical characterization of as-spun composite nanofibers was carried out by X-ray diffraction pattern, field emission scanning electron microscopy, energy-dispersive X-ray spectroscopy, electron probe microanalysis, and transmission electron microscopy, whereas the thermal behavior was analyzed by thermogravimetric analysis. The viability, attachment, and proliferation of NIH 3T3 mouse fibroblast cells on the ZnO/PU composite nanofibers were analyzed by in vitro cell compatibility test. The morphological features of the cells attached on nanofibers were examined by Bio-SEM. We conclude that the electrospun nanofibrous scaffolds with unique spider nets had good biocompatibility. Cytotoxicity experiments indicated that the mouse fibroblasts could attach to the nanocomposite after being cultured. Thus, the current work demonstrates that the as-synthesized ZnO/PU hybrid nanofibers represent a promising biomaterial to be exploited for various tissue engineering applications.
氧化锌（ZnO）纳米结构由于其独特的压电和催化特性，常用于电子学研究；然而，最近它们也被用于生物医学应用。本研究旨在通过一步静电纺丝技术制备 ZnO 掺杂的聚（聚氨酯）（PU）纳米复合材料。所利用的纳米复合材料是由 ZnO 和 PU 组成的胶体凝胶制备而成，所得的纤维垫在 60°C 下真空干燥过夜。通过 X 射线衍射图、场发射扫描电子显微镜、能谱仪、电子探针微分析和透射电子显微镜对纺丝复合材料纳米纤维的物理化学特性进行了表征，而热行为则通过热重分析进行了分析。通过体外细胞相容性试验分析了 NIH 3T3 小鼠成纤维细胞在 ZnO/PU 复合纳米纤维上的生存能力、附着和增殖情况。通过生物扫描电子显微镜（Bio-SEM）检查了附着在纤维上的细胞的形态特征。我们得出结论，具有独特蛛网形态的静电纺纳米纤维支架具有良好的生物相容性。细胞毒性实验表明，经过培养，小鼠成纤维细胞可以附着在纳米复合材料上。因此，目前的工作表明，所合成的 ZnO/PU 杂化纳米纤维代表了一种有前途的生物材料，可用于各种组织工程应用。
