Department of Animal Science, Institute of Rare Earth for Biological Application, Chonbuk National University, Jeonju 561-756, Republic of Korea.
Colloids Surf B Biointerfaces. 2013 Jan 1;101:424-9. doi: 10.1016/j.colsurfb.2012.06.012. Epub 2012 Jun 23.
This study was aimed at the synthesis and characterization of novel Titania nanorods by sol-gel electrospinning technique. The physicochemical properties of the synthesized nanorods were determined by field emission scanning electron microscopy (FE-SEM), energy dispersive X-ray spectroscopy (EDX), transmission electron microscopy (TEM), and X-ray diffraction (XRD) pattern. To examine the in vitro cytotoxicity, mouse myoblast C2C12 cells were treated with different concentrations of as prepared TiO(2) nanorods and the viability of cells was analyzed by Cell Counting Kit-8 assay at regular time intervals. The morphological features of the cells attached with nanorods were examined by Bio-SEM. Cytotoxicity experiments indicated that the mouse myoblast cells could attach to the TiO(2) nanorods after being cultured. We observed that TiO(2) nanorods could support cell adhesion and growth and guide spreading behavior of myoblasts. We conclude that the electrospun TiO(2) nanorods scaffolds with unique morphology had excellent biocompatibility. Thus, the current work demonstrates that the as-synthesized TiO(2) nanorods represent a promising biomaterial to be exploited for various tissue engineering applications.
本研究旨在通过溶胶-凝胶静电纺丝技术合成和表征新型二氧化钛纳米棒。通过场发射扫描电子显微镜（FE-SEM）、能谱（EDX）、透射电子显微镜（TEM）和 X 射线衍射（XRD）图谱来确定合成纳米棒的物理化学性质。为了研究体外细胞毒性，用不同浓度的制备好的 TiO(2)纳米棒处理小鼠成肌细胞 C2C12，并在不同时间间隔通过细胞计数试剂盒-8 分析来分析细胞活力。通过生物扫描电子显微镜（Bio-SEM）来观察与纳米棒附着的细胞的形态特征。细胞毒性实验表明，培养后的小鼠成肌细胞可以附着在 TiO(2)纳米棒上。我们观察到 TiO(2)纳米棒可以支持细胞黏附和生长，并引导成肌细胞的扩展行为。我们得出结论，具有独特形态的静电纺丝 TiO(2)纳米棒支架具有优异的生物相容性。因此，目前的工作表明，所合成的 TiO(2)纳米棒代表了一种有前途的生物材料，可用于各种组织工程应用。
