Centro de Investigaciones sobre Desertificación -CIDE, Moncada, Valencia, Spain.
Anal Bioanal Chem. 2012 Nov;404(9):2659-81. doi: 10.1007/s00216-012-6331-x. Epub 2012 Aug 24.
Although pesticides enable control of the quantity and quality of farm products and food, and help to limit diseases in humans transmitted by insects and rodents, they are regarded as among the most dangerous environmental contaminants because of their tendency to bioaccumulate, and their mobility and long-term effects on living organisms. In the past decade, more analytical methods for accurate identification and quantitative determination of traces of pesticides in biota have been developed to improve our understanding of their risk to ecosystems and humans. Because sample preparation is often the rate-determining step in analysis of pesticides in biological samples, this review first discusses extraction and clean-up procedures, after a brief introduction to the classes, and the methods used in the analysis of pesticides in biota. The analytical methods, especially chromatographic techniques and immunoassay-based methods, are reviewed in detail, and their corresponding advantages, limitations, applications, and prospects are also discussed. This review mainly covers reports published since 2008 on methods for analysis of currently used pesticides in biota.
尽管农药有助于控制农产品和食品的数量和质量，并有助于限制昆虫和啮齿动物传播给人类的疾病，但由于其具有生物累积性、迁移性以及对生物体的长期影响，它们被认为是最危险的环境污染物之一。在过去十年中，为了更准确地识别和定量测定生物体内痕量农药，开发了更多的分析方法，以提高我们对其对生态系统和人类的风险的认识。由于样品制备通常是分析生物样品中农药的决定步骤，因此，在简要介绍农药的类别和分析方法后，本文首先讨论了提取和净化程序。详细审查了分析方法，特别是色谱技术和基于免疫测定的方法，并讨论了它们各自的优点、局限性、应用和前景。本综述主要涵盖了自 2008 年以来发表的关于分析生物体内当前使用的农药的方法的报告。
