MacNeill Stuart
Biomedical Sciences Research Complex, School of Biology, University of St Andrews, North Haugh, St Andrews, Fife, KY16 9ST, UK,
Subcell Biochem. 2012;62:1-17. doi: 10.1007/978-94-007-4572-8_1.
High-fidelity chromosomal DNA replication is vital for maintaining the integrity of the genetic material in all forms of cellular life. In eukaryotic cells, around 40-50 distinct conserved polypeptides are essential for chromosome replication, the majority of which are themselves component parts of a series of elaborate molecular machines that comprise the replication apparatus or replisome. How these complexes are assembled, what structures they adopt, how they perform their functions, and how those functions are regulated, are key questions for understanding how genome duplication occurs. Here I present a brief overview of current knowledge of the composition of the replisome and the dynamic molecular events that underlie chromosomal DNA replication in eukaryotic cells.
高保真染色体DNA复制对于维持所有细胞生命形式中遗传物质的完整性至关重要。在真核细胞中，约40 - 50种不同的保守多肽对染色体复制至关重要，其中大多数本身就是一系列构成复制装置或复制体的复杂分子机器的组成部分。这些复合物如何组装、采用何种结构、如何执行其功能以及这些功能如何被调控，是理解基因组复制如何发生的关键问题。在此，我简要概述一下目前关于真核细胞中复制体组成以及染色体DNA复制基础的动态分子事件的知识。
