Zernike Institute for Advanced Materials, Centre for Synthetic Biology, University of Groningen, 9747 AG Groningen, The Netherlands.
Curr Opin Struct Biol. 2013 Oct;23(5):788-93. doi: 10.1016/j.sbi.2013.06.018. Epub 2013 Jul 24.
Recent advances in the development of single-molecule approaches have made it possible to study the dynamics of biomolecular systems in great detail. More recently, such tools have been applied to study the dynamic nature of large multi-protein complexes that support multiple enzymatic activities. In this review, we will discuss single-molecule studies of the replisome, the protein complex responsible for the coordinated replication of double-stranded DNA. In particular, we will focus on new insights obtained into the dynamic nature of the composition of the DNA-replication machinery and how the dynamic replacement of components plays a role in the regulation of the DNA-replication process.
近年来，单分子方法的发展取得了重大进展，使得人们能够深入研究生物分子系统的动态。最近，这些工具已被应用于研究支持多种酶活性的大型多蛋白复合物的动态性质。在这篇综述中，我们将讨论复制体的单分子研究，复制体是负责双链 DNA 协调复制的蛋白质复合物。特别是，我们将重点介绍对 DNA 复制机制组成的动态性质的新认识，以及组件的动态替换如何在 DNA 复制过程的调控中发挥作用。
