相互作用蛋白质的表面扩散。浓度对吸附牛血清白蛋白横向迁移率的影响。
Surface diffusion of interacting proteins. Effect of concentration on the lateral mobility of adsorbed bovine serum albumin.
作者信息
Tilton R D, Gast A P, Robertson C R
机构信息
Department of Chemical Engineering, Stanford University, California 94305-5025.
出版信息
Biophys J. 1990 Nov;58(5):1321-6. doi: 10.1016/S0006-3495(90)82473-5.
Abstract
Surface diffusion of bovine serum albumin absorbed from aqueous solution to poly(methylmethacrylate) surfaces is significantly hindered by protein-protein lateral interactions. The long-time self diffusion coefficient measured by fluorescence recovery after pattern photobleaching decreases by approximately one order of magnitude as the surface area fraction occupied by protein increases from 0.10 to 0.69. Qualitative features of the surface concentration dependence of the self diffusion coefficient can be described by several recent models for lateral diffusion of interacting species. The mobile fraction is independent of the surface concentration, and both the self diffusion coefficient and the mobile fraction are constant between 15 min and 7 h of adsorption.
摘要
从水溶液吸附到聚甲基丙烯酸甲酯表面的牛血清白蛋白的表面扩散受到蛋白质-蛋白质横向相互作用的显著阻碍。图案光漂白后荧光恢复测量的长时间自扩散系数随着蛋白质占据的表面积分数从0.10增加到0.69而降低约一个数量级。自扩散系数对表面浓度的定性特征可以用最近几个关于相互作用物种横向扩散的模型来描述。可移动部分与表面浓度无关，并且自扩散系数和可移动部分在吸附15分钟到7小时之间是恒定的。
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