冬青匍柄霉（Pedobacter jeongneungensis）sp. nov.，分离自森林土壤。
Pedobacter jeongneungensis sp. nov., isolated from forest soil.
机构信息
Department of Environmental Science and Ecological Engineering, Korea University, Seoul 136-713, Republic of Korea.
出版信息
J Microbiol. 2012 Aug;50(4):660-4. doi: 10.1007/s12275-012-1629-0. Epub 2012 Jul 21.
Strain BH45(T) was isolated from forest soil of Mt. Bukhan in Jeongneung, Seoul, Korea. The Gram-staining-negative strain BH45(T) grows at 4-30°C (optimum of 25-30°C) and between pH 5-8 (optimum of pH 6-8). Its major cellular fatty acids are C(18:3) ω6c (6,9,12) and C(10:0). The G+C content of genomic DNA was 40.2 mol%. The major respiratory quinone system in strain BH45(T) is menaquinone-7. Phylogenetic analysis based on 16S rRNA gene sequences indicates that strain BH45(T) is closely related to the genus Pedobacter. Sequence similarities with P. terrae KCTC 12762(T), P. suwonensis KACC 11317(T), P. soli KACC 14939(T), P. alluvionis DSM 19624(T), P. roseus KCCM 42272(T), P. yonginense KCTC 22721(T) were 97.5, 97.1, 97.0, 97.0, 97.0, and 96.0%, respectively. DNA-DNA hybridization results distinguish strain BH45(T) from two Pedobacter species with high 16S rRNA gene sequence similarities. According to the phenotypic and molecular data, the strain BH45(T) clearly represents a novel species within the genus Pedobacter; thus, the name Pedobacter jeongneungensis sp. nov. is proposed for this strain. The type strain is BH45(T) (=KACC 15514(T) =JCM 17626(T)).
菌株 BH45(T) 是从韩国首尔钟路区北汉山森林土壤中分离得到的。革兰氏阴性菌株 BH45(T) 在 4-30°C（最佳温度为 25-30°C）和 pH5-8（最佳 pH6-8）的条件下生长。其主要细胞脂肪酸为 C(18:3) ω6c (6,9,12) 和 C(10:0)。基因组 DNA 的 G+C 含量为 40.2mol%。菌株 BH45(T) 的主要呼吸醌系统是menaquinone-7。基于 16S rRNA 基因序列的系统发育分析表明，该菌株与 Pedobacter 属密切相关。与 P. terrae KCTC 12762(T)、P. suwonensis KACC 11317(T)、P. soli KACC 14939(T)、P. alluvionis DSM 19624(T)、P. roseus KCCM 42272(T)、P. yonginense KCTC 22721(T) 的 16S rRNA 基因序列相似性分别为 97.5%、97.1%、97.0%、97.0%、97.0%和 96.0%。DNA-DNA 杂交结果表明，与两个具有高 16S rRNA 基因序列相似性的 Pedobacter 种相比，菌株 BH45(T) 存在明显差异。根据表型和分子数据，菌株 BH45(T) 明显代表了 Pedobacter 属中的一个新种；因此，建议将该菌株命名为 Pedobacter jeongneungensis sp. nov.。模式菌株为 BH45(T) (=KACC 15514(T) =JCM 17626(T))。
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