Department of Ministry of Education, Key Laboratory of Child Development and Disorders, Children's Hospital of Chongqing Medical University, Chongqing 400014, PR China.
Mol Med Rep. 2012 Nov;6(5):973-6. doi: 10.3892/mmr.2012.1046. Epub 2012 Aug 24.
The initiation and development of colorectal cancer is closely associated with the malignant transformation of colorectal polyps. The aim of this study was to analyze the expression of the bone morphogenetic protein-2 (BMP2), toll-like receptor 3 (TLR3), TLR4 and cyclooxygenase-2 (COX2) proteins in colorectal polyps, adenoma and adenocarcinoma. An immunohistochemical streptavidin-peroxidase (SP) method was used to examine the expression of MBP2, TLR4, TLR3 and COX2 in 20 colorectal juvenile polyps and 15 colorectal polyps of hamartomatous polyposis obtained from children, and 20 colorectal adenomas and 20 colorectal adenocarcinomas obtained from adults. A comparison of the expression levels of TLR3 among the groups revealed a gradual downward trend from the colorectal juvenile polyp group to the colorectal hamartomatous polyposis, adenoma and adenocarcinoma groups, respectively. The expression level of TLR3 was significantly lower in the colorectal adenocarcinoma group (p<0.05). The expression levels of TLR3, TLR4 and BMP2 were significantly different among the colorectal juvenile polyp, hamartomatous polyposis, adenoma and adenocarcinoma groups. These three protein molecules may be significant in the development and malignant transformation of colorectal polyps.
结直肠癌的发生和发展与结直肠息肉的恶性转化密切相关。本研究旨在分析骨形态发生蛋白-2（BMP2）、Toll 样受体 3（TLR3）、TLR4 和环氧化酶-2（COX2）蛋白在结直肠息肉、腺瘤和腺癌中的表达。采用免疫组织化学链霉亲和素-过氧化物酶（SP）法检测 20 例儿童结直肠幼年性息肉和 15 例结直肠错构瘤性息肉、20 例成人结直肠腺瘤和 20 例结直肠腺癌中 MBP2、TLR4、TLR3 和 COX2 的表达。比较各组 TLR3 的表达水平发现，从结直肠幼年性息肉组到结直肠错构瘤性息肉、腺瘤和腺癌组呈逐渐下降趋势。结直肠腺癌组 TLR3 的表达水平显著降低（p<0.05）。TLR3、TLR4 和 BMP2 的表达水平在结直肠幼年性息肉、错构瘤性息肉、腺瘤和腺癌组之间存在显著差异。这三种蛋白分子可能在结直肠息肉的发生和恶性转化中具有重要意义。
Zotero 插件