Muscle Metabolism Laboratory, Department of Sport and Exercise Science, University of Auckland, Auckland, New Zealand
Exp Physiol. 2013 Feb;98(2):481-90. doi: 10.1113/expphysiol.2012.067603. Epub 2012 Aug 23.
It has been hypothesized that exercise-induced changes in metabolites and ions are crucial in the adaptation of contracting muscle. We tested this hypothesis by comparing adaptations to two different interval-training protocols (differing only in the rest duration between intervals), which provoked different perturbations in muscle metabolites and acid-base status. Prior to and immediately after training, 12 women performed the following tests: (1) a graded exercise test to determine peak oxygen uptake (V(O2)); (2) a high-intensity exercise bout (followed 60 s later by a repeated-sprint-ability test; and (3) a repeat of the high-intensity exercise bout alone with muscle biopsies pre-exercise, immediately postexercise and after 60 s of recovery. Subjects performed 5 weeks (3 days per week) of training, with either a short (1 min; HIT-1) or a long rest period (3 min; HIT-3) between intervals; training intensity and volume were matched. Muscle [H(+)] (155 ± 15 versus 125 ± 8 nmol l(-1); P < 0.05) and muscle lactate content (84.2 ± 7.9 versus 46.9 ± 3.1 mmol (g wet weight)(-1)) were both higher after HIT-1, while muscle phosphocreatine (PCr) content (52.8 ± 8.3 versus 63.4 ± 9.8 mmol (g wet weight)(-1)) was lower. There were no significant differences between the two groups regarding the increases in , repeated-sprint performance or muscle Na(+),K(+)-ATPase content. Following training, both groups had a significant decrease in postexercise muscle [H(+)] and lactate content, but not postexercise ATP or PCr. Postexercise PCr resynthesis increased following both training methods. In conclusion, intense interval training results in marked improvements in muscle Na(+),K(+)-ATPase content, PCr resynthesis and . However, manipulation of the rest period during intense interval training did not affect these changes.
已经有人假设，运动引起的代谢物和离子变化对收缩肌肉的适应至关重要。我们通过比较两种不同的间歇训练方案（仅在间歇之间的休息时间上有所不同）的适应性来检验这一假设，这两种方案引起了肌肉代谢物和酸碱状态的不同干扰。在训练前和训练后立即，12 名女性进行了以下测试：（1）递增运动测试以确定峰值摄氧量（V（O2））；（2）高强度运动回合（随后 60 秒后进行重复冲刺能力测试；（3）在运动前、运动后立即和恢复 60 秒后单独进行高强度运动回合，并进行肌肉活检。受试者进行了 5 周（每周 3 天）的训练，间隔时间分别为短（1 分钟；HIT-1）或长（3 分钟；HIT-3）；训练强度和量相匹配。运动后肌肉[H（+）]（155 ± 15 对 125 ± 8 nmol l（-1）；P < 0.05）和肌肉乳酸含量（84.2 ± 7.9 对 46.9 ± 3.1 mmol（g 湿重）（-1））均在 HIT-1 后更高，而肌肉磷酸肌酸（PCr）含量（52.8 ± 8.3 对 63.4 ± 9.8 mmol（g 湿重）（-1））较低。两组在 增加、重复冲刺表现或肌肉 Na（+）、K（+）-ATPase 含量方面没有显著差异。训练后，两组运动后肌肉[H（+）]和乳酸含量均显著降低，但运动后 ATP 或 PCr 含量没有降低。运动后 PCr 再合成在两种训练方法后均增加。总之，剧烈的间歇训练导致肌肉 Na（+）、K（+）-ATPase 含量、PCr 再合成和 的显著改善。然而，在剧烈间歇训练期间休息时间的操纵并没有影响这些变化。
