Wen Jiagen, Kang Liqun, Liu Huan, Xiao Yiyun, Zhang Xiuzhen, Chen Yuxiang
School of Biological Science and Technology, Central South University, Changsha, People's Republic China.
Pharmacognosy Res. 2012 Jul;4(3):148-53. doi: 10.4103/0974-8490.99076.
Lepidogrammitis drymoglossoides (Baker) Ching (L. drymoglossoides), a member of the Polypodiaceae family, was used in the treatment of numerous diseases. However, none of the potential ingredients and the quality control methods concerning this plant medicine was pronounced.
To identify chlorogenic acid (CGA) from L. drymoglossoides and develop a high performance liquid chromatography (HPLC) assay of CGA.
UV, TLC, and HPLC were utilized to identify the phytochemicals of L. drymoglossoides and determine the CGA content, respectively. The HPLC conditions were as following: a Phenomenex Luna C18 (2) (250 × 4.6 mm i.d.; 5 μm particle size; 100 Å pore size) column; the mobile phase of the mixture of acetonitrile and 0.5% aqueous phosphoric acid (11.5:88.5 v/v); the flow rate of 1.0 mL/min and determination wavelength of 327 nm.
The proposed HPLC method has been developed and validated. The calibration curve was y = 28328x + 16610 (R(2) = 0.9997). The intra-day and inter-day precision and intermediate precision were validated with the RSD less than 5%. The mean recovery rate of the method ranged from 95% to 104%, with the RSD less than 5%. The LOD and LQD values were 0.049 and 0.132 mg/L, respectively. The content of CGA in L. drymoglossoides approximately reached 0.24% (v/v) by the proposed extraction and determination methods.
The assay method was simple, convenient, and accurate to the quantification of CGA and can be used for the quality control of the herb.
石韦属植物庐山石韦（水龙骨科）被用于治疗多种疾病。然而，关于这种植物药的潜在成分和质量控制方法均未明确。
从庐山石韦中鉴定绿原酸（CGA），并建立CGA的高效液相色谱（HPLC）测定法。
分别采用紫外光谱法、薄层色谱法和高效液相色谱法对庐山石韦的植物化学成分进行鉴定并测定CGA含量。HPLC条件如下：使用菲罗门Luna C18(2)柱（250×4.6 mm内径；粒径5μm；孔径100 Å）；流动相为乙腈和0.5%磷酸水溶液的混合物（体积比11.5:88.5）；流速为1.0 mL/min，检测波长为327 nm。
所建立的HPLC方法已得到开发和验证。校准曲线为y = 28328x + 16610（R² = 0.9997）。日内、日间精密度和中间精密度的相对标准偏差（RSD）均小于5%得到验证。该方法的平均回收率在95%至104%之间，RSD小于5%。检测限（LOD）和定量限（LOQ）分别为0.049和0.132 mg/L。通过所提出的提取和测定方法，庐山石韦中CGA的含量约为0.24%（v/v）。
该测定方法简便、准确，可用于CGA的定量分析，并可用于该草药的质量控制。
