Gazit A, Yaniv A, Ianconescu M, Perk K, Aizenberg B, Zimber A
J Virol. 1979 Sep;31(3):639-44. doi: 10.1128/JVI.31.3.639-644.1979.
Recently, we isolated from the blood of lymphoproliferative disease (LPD)-affected turkeys a type C retrovirus distinct from the avian leukosis-sarcoma virus complex and the reticuloendotheliosis virus group. We present molecular evidence for the implication of this virus in the LPD of turkeys. Using complementary DNA of LPD viral RNA, we found that the LPD viral genome is specifically and efficiently transcribed (2,500 copies per cell) in LPD tumor cells. Moreover, the LPD tumor cells contained newly inserted LPD viral information (5 to 10 copies per haploid genome), which was not present before the infection. From the absence of LPD virus-specific sequences in the normal cell genome of turkeys, it was concluded that the LPD virus is not an endogenous virus of turkeys. DNA-DNA annealing experiments revealed that the degree of sequence homology between LPD viral complementary DNA and cellular DNA of turkeys was not higher than that between LPD viral complementary DNA and cellular DNA of other species, thus indicating that the virus does not originate from turkeys.
最近，我们从患有淋巴增生性疾病（LPD）的火鸡血液中分离出一种C型逆转录病毒，它不同于禽白血病-肉瘤病毒复合体和网状内皮组织增殖症病毒群。我们提供了该病毒与火鸡LPD有关的分子证据。利用LPD病毒RNA的互补DNA，我们发现LPD病毒基因组在LPD肿瘤细胞中能特异性且高效地转录（每个细胞2500个拷贝）。此外，LPD肿瘤细胞含有新插入的LPD病毒信息（每个单倍体基因组5到10个拷贝），感染前并不存在。由于在火鸡正常细胞基因组中不存在LPD病毒特异性序列，得出结论：LPD病毒不是火鸡的内源性病毒。DNA-DNA退火实验表明，LPD病毒互补DNA与火鸡细胞DNA之间的序列同源程度不高于LPD病毒互补DNA与其他物种细胞DNA之间的同源程度，因此表明该病毒并非源自火鸡。
