Programa de Mestrado em Ciências Farmacêuticas, Núcleo de Investigações Químico-Farmacêuticas, Universidade do Vale do Itajaí-UNIVALI, Rua Uruguai, 458, Itajaí, Santa Catarina, Brazil.
Naunyn Schmiedebergs Arch Pharmacol. 2012 Nov;385(11):1103-9. doi: 10.1007/s00210-012-0788-1. Epub 2012 Aug 29.
This study was undertaken to evaluate the gastroprotective properties of seed, leaf, and branch methanolic extracts and guttiferone A obtained from Garcinia achachairu (Clusiaceae). Mice were used in all the models, and treatments were administered orally only in pylorus-ligated model of the extracts, and drugs were administered intraduodenally. Treatment with different extracts (500 mg/kg) significantly reduced the ulcerative lesions in the ethanol/HCl-induced model; however, the seed extract was most active. When tested in different doses (50, 250, or 500 mg/kg), the seed extract of G. achaicharu showed a dose-dependent effect with a percentage of inhibition of gastric lesions of 41, 49, and 85 %, respectively. The seed extract also significantly reduced the ulcerative lesions in the indomethacin/bethanechol-induced ulcer. In this model, the percentage of inhibition of ulcer was 24, 58, and 90 %, respectively. Regarding the model of gastric secretion, a reduction of gastric juice volume and total acidity was observed, as well as an increase in gastric pH. Considering that the seed extract was the most active, it was subjected to silica gel column chromatography, leading to the isolation of guttiferone A. The isolated compound and omeprazole were evaluated in the HCl/ethanol-induced ulcer model. In this assay, both compounds at a dose of 30 mg/kg reduced the ulcerative lesions by about 75 %. These results demonstrate, for the first time, that extracts obtained from G. achachairu and guttiferone A produce gastroprotective effects, corroborating ethnomedicinal use of this plant.
这项研究旨在评估来自藤黄属（藤黄科）的种子、叶和枝的甲醇提取物和 guttiferone A 的胃保护特性。所有模型均使用小鼠，仅在提取物的幽门结扎模型中口服给予药物治疗，而在十二指肠内给予药物。不同提取物（500mg/kg）治疗显著减少乙醇/HCl 诱导的模型中的溃疡病变；然而，种子提取物最活跃。当以不同剂量（50、250 或 500mg/kg）测试时，藤黄属 achaicharu 的种子提取物表现出剂量依赖性效应，胃损伤抑制率分别为 41%、49%和 85%。种子提取物还显著减少了吲哚美辛/氨甲酰胆碱诱导的溃疡中的溃疡病变。在该模型中，溃疡抑制率分别为 24%、58%和 90%。关于胃液分泌模型，观察到胃液量和总酸度减少，胃 pH 值增加。考虑到种子提取物最活跃，它被进行硅胶柱色谱分离，得到 guttiferone A。分离得到的化合物和奥美拉唑在盐酸/乙醇诱导的溃疡模型中进行了评估。在该测定中，两种化合物在 30mg/kg 的剂量下均可使溃疡病变减少约 75%。这些结果首次表明，来自藤黄属 achachairu 的提取物和 guttiferone A 产生胃保护作用，证实了该植物的民族医学用途。
