Natural Products and Glycoconjugate Research Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, China; College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China.
Natural Products and Glycoconjugate Research Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, China.
Int J Biol Macromol. 2014 Mar;64:69-75. doi: 10.1016/j.ijbiomac.2013.11.030. Epub 2013 Dec 4.
An immunologically active pectin, named LRGP5, was firstly isolated from the fruits of Lycium ruthenicum Murr. It contained rhamnose, arabinose, xylose, galactose and galacturonic acid in the molar ratio of 1.0:2.2:0.5:1.2:4.7. Its molecular weight was estimated to be approximately 1.37 × 10(5)Da by high performance gel permeation chromatography. The structure was elucidated using methylation analysis, partial acid hydrolysis, NMR and ESI-MS analysis. Results showed that LRGP5 consisted of a (1 → 4)-linked galacturonic acid backbone occasionally interrupted by (1 → 2)-linked rhamnose. The side chains were attached to position 4 of the rhamnosyl units, including (1 → 3)-linked arabinose, (1 → 3)-linked galactose, (1 → 3,6)-linked galactose, (1 → 4)-linked galacturonic acid, (1 → 2)-linked rhamnose and (1 → 2,4)-linked rhamnose, and the termini were arabinose and rhamnose. Immunological assay results demonstrated that LRGP5 could significantly promote macrophage proliferation and enhance the secretion of nitrogen monoxide in vitro.
一种具有免疫活性的多糖 LRGP5 首次从宁夏枸杞果实中分离得到。其糖醛酸摩尔比为 1.0：2.2：0.5：1.2：4.7，由鼠李糖、阿拉伯糖、木糖、半乳糖和半乳糖醛酸组成。其重均分子量通过高效凝胶渗透色谱法估计约为 1.37×10(5)Da。通过甲基化分析、部分酸水解、NMR 和 ESI-MS 分析确定了其结构。结果表明，LRGP5 由（1→4）连接的半乳糖醛酸主链组成，偶尔被（1→2）连接的鼠李糖中断。侧链连接在鼠李糖单元的 4 位，包括（1→3）连接的阿拉伯糖、（1→3）连接的半乳糖、（1→3,6）连接的半乳糖、（1→4）连接的半乳糖醛酸、（1→2）连接的鼠李糖和（1→2,4）连接的鼠李糖，末端为阿拉伯糖和鼠李糖。免疫测定结果表明，LRGP5 能显著促进巨噬细胞增殖，并增强体外一氧化氮的分泌。
