Environment and Health Research laboratory (LERES), Advanced School of Public Health (EHESP), Avenue du Professeur Léon Bernard-CS 74312, 35043 Rennes Cedex, France.
Talanta. 2012 Aug 30;98:157-65. doi: 10.1016/j.talanta.2012.06.064. Epub 2012 Jun 29.
An inter-laboratory exercise was organized under the PHARMAS EU project, by the Advanced School of Public Health (EHESP), in order to evaluate the performances of analytical methods for the measurement of antibiotics in waters (surface and tap). This is the first time such an exercise on antibiotics has been organized in Europe, using different kinds of analytical methods and devices. In this exercise thirteen laboratories from five countries (Canada, France, Italy, the Netherlands and Portugal) participated, and a total number of 78 samples were distributed. During the exercise, 2 testing samples (3 bottles of each) prepared from tap water and river water, respectively, spiked with antibiotics, were sent to participants and analyzed over a period of one month. A final number of 77 (98.7%) testing samples were considered. Depending on substances studied by each participant, 305 values in duplicate were collected, with the results for each sample being expressed as the target concentration. A statistical study was initiated using 611 results. The mean value, standard deviation, coefficient of variation, standard uncertainty of the mean, median, the minimum and maximum values of each series as well as the 95% confidence interval were obtained from each participant laboratory. In this exercise, 36 results (6% of accounted values) were outliers according to the distribution over the median (box plot). The outlier results were excluded. In order to establish the stability of testing samples in the course of the exercise, differences between variances obtained for every type of sample at different intervals were evaluated. The results showed no representative variations and it can be considered that all samples were stable during the exercise. The goals of this inter-laboratory study were to assess results variability when analysis is conducted by different laboratories, to evaluate the influence of different matrix samples, and to determine the rate at which participating laboratories successfully completed the tests initiated.
在 PHARMAS EU 项目的支持下，EHESP 高等公共卫生学院组织了一次实验室间协作研究，以评估抗生素在水中（地表水和自来水）的测量分析方法的性能。这是欧洲首次组织此类涉及多种分析方法和设备的抗生素实验。该实验有来自五个国家（加拿大、法国、意大利、荷兰和葡萄牙）的 13 个实验室参与，共分发了 78 个样本。在实验过程中，参与者收到了 2 个测试样本（各 3 瓶），分别来自自来水和河水，用抗生素进行了加标，然后在一个月的时间内进行分析。最终有 77 个（98.7%）测试样本被认为是有效的。根据每个参与者研究的物质，收集了 305 个重复值，每个样本的结果均表示为目标浓度。随后对 611 个结果进行了统计研究。每个参与者实验室均获得了每个系列的平均值、标准差、变异系数、平均值的标准不确定度、中位数、最小值和最大值以及 95%置信区间。在这次实验中，根据中位数（箱线图）分布，有 36 个结果（占计算值的 6%）是异常值。异常值被排除在外。为了确定实验过程中测试样本的稳定性，评估了不同类型样本在不同时间间隔下获得的方差之间的差异。结果表明没有代表性的变化，可以认为在整个实验过程中所有样本都是稳定的。本次实验室间研究的目的是评估不同实验室进行分析时结果的可变性，评估不同基质样本的影响，并确定参与实验室成功完成初始测试的比例。
