Department of Environmental Sciences, Jozef Stefan Institute, Jamova 39, 1000 Ljubljana, Slovenia.
Environ Pollut. 2010 Mar;158(3):658-62. doi: 10.1016/j.envpol.2009.10.029. Epub 2009 Nov 10.
An inter-laboratory comparison exercise was organized among European laboratories, under the aegis of EU COST Action 636: "Xenobiotics in Urban Water Cycle". The objective was to evaluate the performance of testing laboratories determining "Endocrine Disrupting Compounds" (EDC) in various aqueous matrices. As the main task three steroid estrogens: 17alpha-ethinylestradiol, 17beta-estradiol and estrone were determined in four spiked aqueous matrices: tap water, river water and wastewater treatment plant influent and effluent using GC-MS and LC-MS/MS. Results were compared and discussed according to the analytical techniques applied, the accuracy and reproducibility of the analytical methods and the nature of the sample matrices. Overall, the results obtained in this inter-laboratory exercise reveal a high level of competence among the participating laboratories for the detection of steroid estrogens in water samples indicating that GC-MS as well as LC-MS/MS can equally be employed for the analysis of natural and synthetic hormones.
在欧盟 COST 行动 636“城市水环中的外源化合物”的支持下，欧洲的实验室之间组织了一次实验室间的比对活动。该活动的目的是评估测试实验室在各种水基质中测定“内分泌干扰化合物”（EDC）的性能。作为主要任务，使用 GC-MS 和 LC-MS/MS 对自来水中、河水中以及污水处理厂进水和出水这四种加标水基质中的三种甾体雌激素：17α-乙炔雌二醇、17β-雌二醇和雌酮进行了测定。根据所应用的分析技术、分析方法的准确性和重现性以及样品基质的性质对结果进行了比较和讨论。总的来说，该实验室间活动的结果表明，参与实验室在水中甾体雌激素的检测方面具有较高的能力，这表明 GC-MS 和 LC-MS/MS 均可用于天然和合成激素的分析。
