Gomella L G, Anglard P, Sargent E R, Robertson C N, Kasid A, Linehan W M
Surgery Branch, National Cancer Institute, Bethesda, Maryland.
J Urol. 1990 Jan;143(1):191-3. doi: 10.1016/s0022-5347(17)39908-1.
The epidermal growth factor receptor binds the mitogens epidermal growth factor and transforming growth factor-alpha. Increased expression of the epidermal growth factor receptor has been noted in many types of tumors and is associated with gene amplification in several including epidermoid carcinoma, lung carcinoma, breast carcinoma and glioblastoma. We have recently observed increased expression of the epidermal growth factor receptor messenger RNA in neoplastic tissue relative to normal kidney tissue from patients with renal cell carcinoma. To determine if epidermal growth factor receptor gene amplification was present in renal cell carcinoma, DNA was extracted from renal cell carcinoma cell lines and from normal kidney and renal cell carcinoma tissues derived from radical nephrectomy specimens from thirty patients. DNA was analyzed by Southern blot hybridization. There was no epidermal growth factor receptor gene amplification detected in the renal cell carcinoma samples studied, indicating the increased epidermal growth factor gene expression observed in renal cell carcinoma does not occur through gene amplification. Unlike other tumors with enhanced epidermal growth factor receptor gene expression, amplification of this gene does not appear to be a common feature of renal cell carcinoma.
表皮生长因子受体可结合有丝分裂原表皮生长因子和转化生长因子-α。在多种肿瘤中已观察到表皮生长因子受体表达增加，且在包括表皮样癌、肺癌、乳腺癌和胶质母细胞瘤在内的几种肿瘤中与基因扩增相关。我们最近观察到，与肾细胞癌患者的正常肾组织相比，肿瘤组织中表皮生长因子受体信使核糖核酸的表达增加。为了确定肾细胞癌中是否存在表皮生长因子受体基因扩增，从肾细胞癌细胞系以及30例患者根治性肾切除标本所获的正常肾组织和肾细胞癌组织中提取DNA。通过Southern印迹杂交分析DNA。在所研究的肾细胞癌样本中未检测到表皮生长因子受体基因扩增，这表明在肾细胞癌中观察到的表皮生长因子基因表达增加并非通过基因扩增发生。与其他表皮生长因子受体基因表达增强的肿瘤不同，该基因的扩增似乎并非肾细胞癌的常见特征。
