SeqTrace：一种用于快速处理DNA测序色谱图的图形工具。
SeqTrace: a graphical tool for rapidly processing DNA sequencing chromatograms.
作者信息
Stucky Brian J
机构信息
Department of Ecology and Evolutionary Biology, University of Colorado, Boulder, Colorado 80309, USA.
出版信息
J Biomol Tech. 2012 Sep;23(3):90-3. doi: 10.7171/jbt.12-2303-004.
Modern applications of Sanger DNA sequencing often require converting a large number of chromatogram trace files into high-quality DNA sequences for downstream analyses. Relatively few nonproprietary software tools are available to assist with this process. SeqTrace is a new, free, and open-source software application that is designed to automate the entire workflow by facilitating easy batch processing of large numbers of trace files. SeqTrace can identify, align, and compute consensus sequences from matching forward and reverse traces, filter low-quality base calls, and end-trim finished sequences. The software features a graphical interface that includes a full-featured chromatogram viewer and sequence editor. SeqTrace runs on most popular operating systems and is freely available, along with supporting documentation, at http://seqtrace.googlecode.com/.
桑格DNA测序的现代应用通常需要将大量的色谱图跟踪文件转换为高质量的DNA序列，以便进行下游分析。可用于协助此过程的非专有软件工具相对较少。SeqTrace是一款全新的免费开源软件应用程序，旨在通过方便地批量处理大量跟踪文件来实现整个工作流程的自动化。SeqTrace可以从匹配的正向和反向跟踪中识别、比对并计算一致序列，过滤低质量的碱基调用，并对完成的序列进行末端修剪。该软件具有图形界面，其中包括功能齐全的色谱图查看器和序列编辑器。SeqTrace可在大多数流行的操作系统上运行，并且可以在http://seqtrace.googlecode.com/上免费获取，同时还提供支持文档。
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