Engelman R W, Day N K, Chen R F, Tomita Y, Bauer-Sardiña I, Dao M L, Good R A
Department of Pediatrics, All Children's Hospital, University of South Florida, St. Petersburg 33701.
Proc Soc Exp Biol Med. 1990 Jan;193(1):23-30. doi: 10.3181/00379727-193-42984.
To analyze simultaneously the influence attributable to calorie consumption level and percentage of dietary fat on the spontaneous development of mammary adenocarcinoma, virgin female C3H/Ou mice were separated into five dietary groups. Four groups of mice were fed purified diets either ad libitum (16-18 kcal/mouse/day) or restricted 40% in calorie consumption (10-11 kcal/mouse/day), and diets contained either 4.5%, 7.5%, 67%, or 68% calories from fat. Mice that consumed isocaloric diets developed breast malignancy at a comparable pace. Consuming a diet in which fats were present only at levels sufficient to satisfy the threshold requirement of essential fatty acids, 4.5-7.5% of the total calories, or alternatively where dietary fat represented greater than 67% of the total calories consumed, did not significantly alter the tendency for breast tumor development. The pace and frequency with which tumors occurred reflected the host's level of calorie consumption. Mice consuming a high caloric diet, low or high in fat, tended to have a shortened latency to breast tumor formation, an increased incidence of breast tumors, elevated serum prolactin levels, elevated levels of antibodies to mouse mammary tumor virus, and elevated circulating immune complex levels.
为了同时分析卡路里摄入量和膳食脂肪百分比对乳腺腺癌自发发展的影响，将处女雌性C3H/Ou小鼠分为五个饮食组。四组小鼠分别随意进食纯化饮食（16 - 18千卡/小鼠/天）或卡路里摄入量限制40%（10 - 11千卡/小鼠/天），饮食中脂肪提供的热量分别为4.5%、7.5%、67%或68%。摄入等热量饮食的小鼠乳腺恶性肿瘤的发展速度相当。食用脂肪含量仅足以满足必需脂肪酸阈值需求（占总热量的4.5 - 7.5%）的饮食，或者膳食脂肪占总热量超过67%的饮食，均未显著改变乳腺肿瘤发展的趋势。肿瘤发生的速度和频率反映了宿主的卡路里摄入水平。食用高脂肪或低脂肪高热量饮食的小鼠，乳腺肿瘤形成的潜伏期往往缩短，乳腺肿瘤发病率增加，血清催乳素水平升高，抗小鼠乳腺肿瘤病毒抗体水平升高，循环免疫复合物水平升高。
