Stark R, Rümenapf T, Meyers G, Thiel H J
Federal Research Centre for Virus Diseases of Animals, Tübingen, Federal Republic of Germany.
Virology. 1990 Jan;174(1):286-9. doi: 10.1016/0042-6822(90)90076-4.
A polyspecific antiserum has been used to identify four different glycoproteins in hog cholera virus (HCV)-infected cells termed gp55, gp48, gp44, and gp33 (Rümenapf et al, 1989, Virology 171, 18-27). Fusion proteins containing parts of the putative HCV-encoded glycoproteins were expressed in bacteria and served for the preparation of specific antibodies. These were used in radioimmunoprecipitation assays which revealed that gp48 and gp44 most likely share a common protein backbone. The order of the glycoproteins on the HCV genome was determined as follows: NH2-gp44/gp48-gp33-gp55-COOH.
一种多特异性抗血清已被用于鉴定猪霍乱病毒（HCV）感染细胞中的四种不同糖蛋白，分别称为gp55、gp48、gp44和gp33（吕梅纳普夫等人，1989年，《病毒学》171卷，第18 - 27页）。含有部分假定的HCV编码糖蛋白的融合蛋白在细菌中表达，并用于制备特异性抗体。这些抗体用于放射免疫沉淀测定，结果显示gp48和gp44很可能共享一个共同的蛋白质骨架。HCV基因组上糖蛋白的顺序确定如下：NH2 - gp44/gp48 - gp33 - gp55 - COOH。
