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诱导中和抗体的瘟病毒糖蛋白是二硫键连接的异源二聚体的一部分。

Pestivirus glycoprotein which induces neutralizing antibodies forms part of a disulfide-linked heterodimer.

作者信息

Weiland E, Stark R, Haas B, Rümenapf T, Meyers G, Thiel H J

机构信息

Federal Research Centre for Virus Diseases of Animals, Tübingen, Federal Republic of Germany.

出版信息

J Virol. 1990 Aug;64(8):3563-9. doi: 10.1128/JVI.64.8.3563-3569.1990.

DOI:10.1128/JVI.64.8.3563-3569.1990
PMID:2370675
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC249648/
Abstract

Neutralizing monoclonal antibodies directed against hog cholera virus (HCV) precipitated two HCV-encoded glycoproteins, HCV gp55 and HCV gp33. Immunoassay with bacterial fusion proteins and Western immunoblotting with extracts from infected cells revealed that the antibodies recognized only HCV gp55. Coprecipitation of HCV gp33 was shown to be due to intermolecular disulfide bridges. One of the antibodies also reacted with the major glycoprotein of another pestivirus, bovine viral diarrhea virus (BVDV). The analogous BVDV glycoproteins exhibited a distribution of cysteine residues which was almost identical to that of HCV gp55 and gp33. The two BVDV glycoproteins were also linked by disulfide bridges.

摘要

针对猪瘟病毒（HCV）的中和单克隆抗体沉淀出两种HCV编码的糖蛋白，即HCV gp55和HCV gp33。用细菌融合蛋白进行免疫测定以及用感染细胞提取物进行Western免疫印迹分析表明，这些抗体仅识别HCV gp55。已证明HCV gp33的共沉淀是由于分子间二硫键所致。其中一种抗体还与另一种瘟病毒——牛病毒性腹泻病毒（BVDV）的主要糖蛋白发生反应。类似的BVDV糖蛋白的半胱氨酸残基分布与HCV gp55和gp33几乎相同。这两种BVDV糖蛋白也通过二硫键相连。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dfe8/249648/1def3a6da67a/jvirol00063-0014-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dfe8/249648/2c17196f8a1d/jvirol00063-0012-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dfe8/249648/51a87f4c6713/jvirol00063-0013-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dfe8/249648/2b7ebdd3355d/jvirol00063-0013-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dfe8/249648/963f8edd0a51/jvirol00063-0014-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dfe8/249648/1def3a6da67a/jvirol00063-0014-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dfe8/249648/2c17196f8a1d/jvirol00063-0012-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dfe8/249648/51a87f4c6713/jvirol00063-0013-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dfe8/249648/2b7ebdd3355d/jvirol00063-0013-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dfe8/249648/963f8edd0a51/jvirol00063-0014-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dfe8/249648/1def3a6da67a/jvirol00063-0014-b.jpg

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