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本文引用的文献

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Cellular immune response to hog cholera virus (HCV): T cells of immune pigs proliferate in vitro upon stimulation with live HCV, but the E1 envelope glycoprotein is not a major T-cell antigen.对猪霍乱病毒（HCV）的细胞免疫反应：免疫猪的T细胞在受到活HCV刺激后在体外增殖，但E1包膜糖蛋白不是主要的T细胞抗原。
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Processing of the envelope glycoproteins of pestiviruses.瘟病毒包膜糖蛋白的加工过程。
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Identification of a structural glycoprotein of an RNA virus as a ribonuclease.鉴定一种RNA病毒的结构糖蛋白为核糖核酸酶。
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Glycoprotein E1 of hog cholera virus expressed in insect cells protects swine from hog cholera.在昆虫细胞中表达的猪霍乱病毒糖蛋白E1可保护猪免受猪霍乱的侵害。
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Glycoprotein E2 of classical swine fever virus: expression in insect cells and identification as a ribonuclease.经典猪瘟病毒糖蛋白E2：在昆虫细胞中的表达及作为核糖核酸酶的鉴定
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Biology of cloned cytotoxic T lymphocytes specific for lymphocytic choriomeningitis virus: clearance of virus in vivo.针对淋巴细胞性脉络丛脑膜炎病毒的克隆化细胞毒性T淋巴细胞的生物学特性：病毒在体内的清除
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10
Identification of the human cytomegalovirus glycoprotein B gene and induction of neutralizing antibodies via its expression in recombinant vaccinia virus.人巨细胞病毒糖蛋白B基因的鉴定及其在重组痘苗病毒中的表达诱导中和抗体
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经典猪瘟病毒：两种结构糖蛋白独立诱导保护性免疫

Classical swine fever virus: independent induction of protective immunity by two structural glycoproteins.

作者信息

König M, Lengsfeld T, Pauly T, Stark R, Thiel H J

机构信息

Federal Research Centre for Virus Diseases of Animals, Tübingen, Germany.

出版信息

J Virol. 1995 Oct;69(10):6479-86. doi: 10.1128/JVI.69.10.6479-6486.1995.

DOI:10.1128/JVI.69.10.6479-6486.1995
PMID:7666549
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC189549/
Abstract

To study which proteins of classical swine fever virus (CSFV) are able to confer protective immunity in swine, N-terminal autoprotease, viral core protein, and the three structural glycoproteins were expressed via vaccinia virus recombinants (VVR). CSFV proteins synthesized in cells infected with VVR showed migration characteristics on sodium dodecyl sulfate gels identical to those of their respective CSFV counterparts. Apparently authentic dimerization of the recombinant glycoproteins was observed. The glycoproteins E0 and E2 were detected on the surfaces of VVR-infected cells. In protection experiments, swine were immunized with the different VVR, and the generation of humoral immune response was monitored. Only animals vaccinated with VVR expressing E0 and/or E2 resisted a lethal challenge infection with CSFV. Glycoprotein E0 represents a second determinant for the induction of protective immunity against classical swine fever.

摘要

为研究经典猪瘟病毒（CSFV）的哪些蛋白能够在猪体内产生保护性免疫，通过痘苗病毒重组体（VVR）表达了N端自蛋白酶、病毒核心蛋白和三种结构糖蛋白。在感染VVR的细胞中合成的CSFV蛋白在十二烷基硫酸钠凝胶上显示出与其各自CSFV对应物相同的迁移特征。观察到重组糖蛋白明显发生了真实的二聚化。在VVR感染细胞的表面检测到了糖蛋白E0和E2。在保护实验中，用不同的VVR对猪进行免疫，并监测体液免疫反应的产生。只有接种了表达E0和/或E2的VVR的动物能够抵抗CSFV的致死性攻击感染。糖蛋白E0是诱导针对经典猪瘟的保护性免疫的第二个决定因素。