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A convenient sequencing method for 5' protein-linked RNAs.

作者信息

Nomoto A, Imura N

出版信息

Nucleic Acids Res. 1979 Nov 10;7(5):1233-46. doi: 10.1093/nar/7.5.1233.

DOI:10.1093/nar/7.5.1233
PMID:229466
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC342299/
Abstract

A convenient nucleotide sequencing method for 5' end protein-linked RNAs was developed. Genome of LSc, 2ab poliovirus, which has a protein (VPg) covalently linked to the 5' terminus, was labelled with 125I Bolton and Hunter reagent after proteinase K treatment. No sign of labelling of nucleotide moiety in the genome with the reagent was detected. A labelled oligo peptide-linked ribonuclease T1 fragment was obtained from the 5' end of the genome. Analysis of the complex by two dimensional gel electrophoresis after partial alkali digestion or by the nucleotide sequencing method of Donis-Keller et al. (1) revealed that LSc, 2ab strain genome had an identical 5' end structure to that of Mahoney strain genome, that is, VPg-pUpUpApApApApCpApGp. Our results have shown that this labelling method is useful for analysis of 5' end sequence of RNAs linked to protein at the 5' termini.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0a7/342299/dc9fed54ebad/nar00458-0139-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0a7/342299/655e215935c1/nar00458-0134-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0a7/342299/2650911026ce/nar00458-0136-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0a7/342299/c784d5f6f440/nar00458-0138-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0a7/342299/dc9fed54ebad/nar00458-0139-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0a7/342299/655e215935c1/nar00458-0134-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0a7/342299/2650911026ce/nar00458-0136-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0a7/342299/c784d5f6f440/nar00458-0138-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0a7/342299/dc9fed54ebad/nar00458-0139-a.jpg

相似文献

1
A convenient sequencing method for 5' protein-linked RNAs.
Nucleic Acids Res. 1979 Nov 10;7(5):1233-46. doi: 10.1093/nar/7.5.1233.
2
Possible point mutation sites in LSc, 2ab poliovirus RNA and a protein covalently linked to the 5'-terminus.脊髓灰质炎病毒LSc、2ab型RNA以及与5'-末端共价连接的一种蛋白质中可能的点突变位点。
J Gen Virol. 1979 Oct;45(1):107-17. doi: 10.1099/0022-1317-45-1-107.
3
The genome-linked protein of picornaviruses. VI. The 5'-terminal protein of poliovirus type 2RNA is covalently linked to a nonanucleotide identical to that of poliovirus type 1 RNA.小核糖核酸病毒的基因组连接蛋白。VI. 2型脊髓灰质炎病毒RNA的5'末端蛋白与一个与1型脊髓灰质炎病毒RNA的九核苷酸相同的九核苷酸共价连接。
Intervirology. 1980;13(3):192-9. doi: 10.1159/000149125.
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Identification of specific fragments containing the 5' end of poliovirus RNA after ribonuclease III digestion.核糖核酸酶III消化后含脊髓灰质炎病毒RNA 5'端的特定片段的鉴定
Nucleic Acids Res. 1978 Nov;5(11):4039-54. doi: 10.1093/nar/5.11.4039.
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Identification of point mutations in the genome of the poliovirus Sabin vaccine LSc 2ab, and catalogue of RNase T1- and RNase A-resistant oligonucleotides of poliovirus type 1 (Mahoney) RNA.脊髓灰质炎病毒萨宾疫苗LSc 2ab基因组中位点突变的鉴定，以及1型脊髓灰质炎病毒（马奥尼株）RNA的核糖核酸酶T1和核糖核酸酶A抗性寡核苷酸目录。
Virology. 1981 Jul 15;112(1):217-27. doi: 10.1016/0042-6822(81)90627-9.
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Covalent linkage of a protein to a defined nucleotide sequence at the 5'-terminus of virion and replicative intermediate RNAs of poliovirus.脊髓灰质炎病毒的病毒体和复制中间体RNA的5'-末端，蛋白质与特定核苷酸序列的共价连接。
Proc Natl Acad Sci U S A. 1977 Mar;74(3):961-5. doi: 10.1073/pnas.74.3.961.
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Determination of the length distribution of poly(A) at the 3' terminus of the virion RNAs of EMC virus, poliovirus, rhinovirus, RAV-61 and CPMV and of mouse globin mRNA.测定脑心肌炎病毒、脊髓灰质炎病毒、鼻病毒、RAV - 61和菜豆金色花叶病毒的病毒粒子RNA以及小鼠珠蛋白mRNA 3'端多聚腺苷酸的长度分布。
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[Detection of a protein firmly bound to the RNA of radish mosaic virus].
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Sequence of picornavirus RNAs containing a radioiodinated 5'-linked peptide reveals a conserved 5' sequence.含有放射性碘化5'-连接肽的微小核糖核酸病毒RNA序列揭示了一个保守的5'序列。
Proc Natl Acad Sci U S A. 1980 Jan;77(1):303-7. doi: 10.1073/pnas.77.1.303.
10
Nucleotide sequences adjacent to the proteins covalently linked to the cowpea mosaic virus genome. Sequence determination after labelling in vitro using RNA ligase.与共价连接到豇豆花叶病毒基因组的蛋白质相邻的核苷酸序列。使用RNA连接酶进行体外标记后的序列测定。
Eur J Biochem. 1979 Nov 1;101(1):45-9. doi: 10.1111/j.1432-1033.1979.tb04214.x.

引用本文的文献

1
Stable hairpin structure within the 5'-terminal 85 nucleotides of poliovirus RNA.脊髓灰质炎病毒RNA 5'端85个核苷酸内的稳定发夹结构。
J Virol. 1981 Jan;37(1):328-35. doi: 10.1128/JVI.37.1.328-335.1981.
2
The 5'-end sequence of the murine coronavirus genome: implications for multiple fusion sites in leader-primed transcription.鼠冠状病毒基因组的5'端序列：对引导引物转录中多个融合位点的影响
Virology. 1987 Feb;156(2):321-30. doi: 10.1016/0042-6822(87)90412-0.

本文引用的文献

1
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
2
The labelling of proteins to high specific radioactivities by conjugation to a 125I-containing acylating agent.通过与含¹²⁵I的酰化剂结合将蛋白质标记至高比放射性。
Biochem J. 1973 Jul;133(3):529-39. doi: 10.1042/bj1330529.
3
A sequence of 50 nucleotides from coliphage R17 RNA.来自大肠杆菌噬菌体R17 RNA的一段50个核苷酸的序列。
Eur J Biochem. 1973 Feb 15;33(1):8-18. doi: 10.1111/j.1432-1033.1973.tb02648.x.
4
Virus-induced interference in heterologously infected HeLa cells.病毒诱导的对异源感染的HeLa细胞的干扰。
J Virol. 1972 Jan;9(1):22-8. doi: 10.1128/JVI.9.1.22-28.1972.
5
Regulation of protein synthesis in HeLa cells. 3. Inhibition during poliovirus infection.HeLa细胞中蛋白质合成的调控。3. 脊髓灰质炎病毒感染期间的抑制作用。
J Virol. 1971 Nov;8(5):661-8. doi: 10.1128/JVI.8.5.661-668.1971.
6
Purification of the Sabin strain of poliovirus type I through treatment with sarkozyl.通过用 Sarkozyl 处理来纯化脊髓灰质炎病毒 I 型的萨宾株。
J Virol. 1971 May;7(5):687-9. doi: 10.1128/JVI.7.5.687-689.1971.
7
Evidence for role of m7G5'-phosphate group in recognition of eukaryotic mRNA by initiation factor IF-M3.起始因子IF-M3识别真核生物mRNA过程中m7G5'-磷酸基团作用的证据。
Nature. 1976 May 27;261(5558):291-4. doi: 10.1038/261291a0.
8
Cowpea mosaic virus RNAs have neither m7GpppN ... nor mono-, di- or triphosphates at their 5' ends.豇豆花叶病毒RNA在其5'末端既没有m7GpppN……也没有一磷酸、二磷酸或三磷酸。
Cell. 1977 May;11(1):73-82. doi: 10.1016/0092-8674(77)90318-x.
9
Presence of a covalently linked protein on calicivirus RNA.杯状病毒RNA上存在共价连接的蛋白质。
J Gen Virol. 1978 Nov;41(2):443-6. doi: 10.1099/0022-1317-41-2-443.
10
Mapping adenines, guanines, and pyrimidines in RNA.绘制RNA中的腺嘌呤、鸟嘌呤和嘧啶。
Nucleic Acids Res. 1977 Aug;4(8):2527-38. doi: 10.1093/nar/4.8.2527.