Selseleh Mina, Keshavarz Hossein, Mohebali Mehdi, Shojaee Saeedeh, Selseleh Monavar, Eshragian Mohammad Reza, Mansouri Fatemeh, Modarressi Mohammad Hossein
Department of Medical Parasitology and Mycology, School of Public Health (Affiliation Center), Tehran University of Medical Sciences, Tehran, Iran.
Korean J Parasitol. 2012 Sep;50(3):233-8. doi: 10.3347/kjp.2012.50.3.233. Epub 2012 Aug 13.
The precise diagnosis of the acute toxoplasmosis in pregnant women and immunocompromsied patients has critical importance. Most of the commercially available assays use the whole Toxoplasma soluble extract as the antigen. However, the assays currently available for the detection of specific anti-Toxoplasma antibodies may vary in their abilities to detect serum immunoglobulins, due to the lack of a purified standardized antigen. The aim of this study was production and evaluation of the usefulness of the recombinant Toxoplasma gondii GRA7 antigen for the serodiagnosis of Toxoplasma gondii IgM and IgG by ELISA. A total of 70 T. gondii IgM positive sera, 74 T. gondii IgG positive sera, and 60 sera from subjects who were not infected with T. gondii were examined. These sera were shown different absorbance values in ELISA test. To control the specificity of the rGRA7 other parasitic diseases, for example, echinococcosis, malaria, leishmaniasis, fascioliasis, and strongyloidiasis were tested of which none showed positive results. Sensitivity and specificity of the generated recombinant IgG ELISA in comparison with commercial ELISA (com ELISA) were 89% and 90%, and the sensitivity and specificity of the generated recombinant IgM ELISA were 96% and 90%, respectively. The results obtained here show that this antigen is useful for diagnostic purposes.
准确诊断孕妇和免疫功能低下患者的急性弓形虫病至关重要。大多数市售检测方法使用整个弓形虫可溶性提取物作为抗原。然而，由于缺乏纯化的标准化抗原，目前用于检测特异性抗弓形虫抗体的检测方法在检测血清免疫球蛋白的能力上可能存在差异。本研究的目的是生产重组弓形虫GRA7抗原，并通过ELISA评估其在弓形虫IgM和IgG血清学诊断中的实用性。共检测了70份弓形虫IgM阳性血清、74份弓形虫IgG阳性血清以及60份未感染弓形虫受试者的血清。这些血清在ELISA检测中显示出不同的吸光度值。为控制rGRA7对其他寄生虫病（如棘球蚴病、疟疾、利什曼病、片形吸虫病和粪类圆线虫病）的特异性，对这些疾病进行了检测，结果均为阴性。与商业ELISA（com ELISA）相比，所制备的重组IgG ELISA的敏感性和特异性分别为89%和90%，重组IgM ELISA的敏感性和特异性分别为96%和90%。此处获得的结果表明，该抗原可用于诊断目的。
