Chua Lee Suan, Alitabarimansor Meisam, Lee Chew Tin, Mat Ramli
Metabolites Profiling Laboratory, Institute of Bioproduct Development, Universiti Teknologi Malaysia, Johor, 81310 Johor Bahru, Malaysia.
Enzyme Res. 2012;2012:542589. doi: 10.1155/2012/542589. Epub 2012 Aug 16.
Hydrolysis of virgin coconut oil (VCO) had been carried out by using an immobilised lipase from Mucor miehei (Lipozyme) in a water-jacketed batch reactor. The kinetic of the hydrolysis was investigated by varying the parameters such as VCO concentration, enzyme loading, water content, and reaction temperature. It was found that VCO exhibited substrate inhibition at the concentration more than 40% (v/v). Lipozyme also achieved the highest production of free fatty acids, 4.56 mM at 1% (w/v) of enzyme loading. The optimum water content for VCO hydrolysis was 7% (v/v). A relatively high content of water was required because water was one of the reactants in the hydrolysis. The progress curve and the temperature profile of the enzymatic hydrolysis also showed that Lipozyme could be used for free fatty acid production at the temperature up to 50°C. However, the highest initial reaction rate and the highest yield of free fatty acid production were at 45 and 40°C, respectively. A 100 hours of initial reaction time has to be compensated in order to obtain the highest yield of free fatty acid production at 40°C.
在夹套式间歇反应器中，使用米黑根毛霉固定化脂肪酶（Lipozyme）对初榨椰子油（VCO）进行水解。通过改变VCO浓度、酶负载量、含水量和反应温度等参数来研究水解动力学。结果发现，当VCO浓度超过40%（v/v）时会出现底物抑制现象。在酶负载量为1%（w/v）时，Lipozyme也实现了游离脂肪酸的最高产量，为4.56 mM。VCO水解的最佳含水量为7%（v/v）。由于水是水解反应的反应物之一，因此需要相对较高的含水量。酶促水解的进程曲线和温度曲线还表明，Lipozyme可用于在高达50°C的温度下生产游离脂肪酸。然而，最高初始反应速率和游离脂肪酸生产的最高产率分别出现在45°C和40°C。为了在40°C下获得游离脂肪酸生产的最高产率，必须补偿100小时的初始反应时间。
